南方医科大学学报2012，Vol.32Issue(5)：710-713,4.DOI:10.3969/j.issn.1673-4254.2012.05.028
1,25-二羟维生素D3对胃癌细胞增殖和TNF-α表达的影响
Effects of 1,25(OH)2D3 on SGC-7901 cell proliferation and tumor necrosis factor-α expression
摘要
Abstract
Objective To investigate the effect of 1,25 (OH)2D3 on cell proliferation and the expression of tumor necrosis factor-α (TNF-α) in human gastric carcinoma SGC-7901 cells.Methods SGC-7901 cells were treated with 1×107,1×107,l×107,and l×107 mol/L 1,25 (OH)2D3 for 24,48,72 and 96 h.The cell proliferation was measured by MTT assay,and the cell cycle changes were analyzed using flow cytometry.RT-PCK and Western blotting were used to determine the expression of TNF-α mRNA and protein,respectively.Results 1,25 (OH)2D3 significantly inhibited SGC-7901 cell proliferation (P<0.05) in a time- and dose-dependent fashion.Treatment with 1,25 (OH)2D3 for 72 h caused significant cell cycle arrest at G0/G1 phase (F=9.81,P< 0.05) and dose-dependently inhibited the expression of TNF-a at both mRNA and protein levels in SGC-7901 cells (P<0.05).Conclusion The inhibitory effect of 1,25 (OH)2D3 on SGC-7901 cell proliferation is probably associated with the down-regulation of TNF-α expression.关键词
1,25-二羟维生素D3/胃肿瘤/细胞增殖/细胞周期/肿瘤坏死因子-αKey words
1,25-dihydroxy-vitamin D2/stomach neoplasm/cell proliferation/rumor necrosis factor-α分类
医药卫生引用本文复制引用
张莉,王璐璐,吴小翎..1,25-二羟维生素D3对胃癌细胞增殖和TNF-α表达的影响[J].南方医科大学学报,2012,32(5):710-713,4.基金项目
重庆市卫生局医学科研项目(2008-1-26) （2008-1-26）
