南方医科大学学报2012，Vol.32Issue(5)：726-729,733,5.DOI:10.3969/j.issn.1673-4254.2012.05.032
2型糖尿病合并非酒精性脂肪肝大鼠肝脏SIRT1、UCP2表达的变化
Hepatic SIRT1 and UCP2 expressions in rats with type 2 diabetes mellitus and nonalcoholic fatty liver
摘要
Abstract
Objective To observe the expression of SIRT1 and mitochondrial uncoupling protein 2 (UCP2) in the liver of rats with type 2 diabetes mellirus (T2DM) and nonalcoholic fatty liver (NAFLD) and explore the possible pathogenesis of T2DM and NAFLD.Methods Twenty-four male SD rat were randomized equally into control group and T2DM and NAFLD group (MC group),fed with standard diet and high-fat and high-sugar diet,respectively- At 12 weeks,(he rats in MC group received a single dose of ST2 (30 mg/kg) injected into the abdominal cavity for pancreatic islet destruction,and those in the control group received an equivalent volume of citric acid buffer.At 14 weeks,the body weight,FBG,hepatic function,blood lipid levels,FFAs,FINs and HOMA-1R of the rats were measured,and the liver pathology was examined with HE staining.The expression of 51RT1 and UCP2 in the rat liver was detected by immunohistochemistry and real-time quantitative PCR.Results At 14 weeks,FBG,ALT,AST,TC,TG,LDL-C,VLDL,FFAs,FINs and HOMA-IR were significantly higher and HDL-C was significantly lower in MC group than in the control group (P0.05).Pathological examination showed good structural integrity of the liver in the control group,and the liver cells were closely arranged with rich cytoplasm and round cell nuclei: in MC group,moderate to severe fatty liver was detected,and the liver cells showed severe ballooning degeneration and contained lipid vacuoles in the cytoplasm.The expression of SIRT1 was significantly lower and UCP2 significantly higher in MC group than in the control group (P<0.05).Conclusion The expression of S1RT1 is significantly lowered and UCP2 increased in the liver of rats with T2DM and NAFLD.关键词
沉默调节蛋白1/线粒体脱偶连蛋白2/2型糖尿病/非酒精性脂肪肝Key words
SIRT1/mitochondrial uncoupling protein 2/type 2 diabetes mellirus/nonalcoholic fatty liver分类
医药卫生引用本文复制引用
徐静,李楠,王俊红,张春虹,丁石梅,焦杨..2型糖尿病合并非酒精性脂肪肝大鼠肝脏SIRT1、UCP2表达的变化[J].南方医科大学学报,2012,32(5):726-729,733,5.基金项目
陕西省科技计划项目[2007K14-04(4)] （4）
