南方医科大学学报2012，Vol.32Issue(7)：919-923,5.DOI:10.3969/j.issn.1673-4254.2012.07.002
抗人卵巢癌/抗人CD3单链双特异性抗体介导的αβT细胞CDR3谱系漂移
An anti-human ovarian carcinoma and CD3 bispecific single-chain antibody mediates CDR3 spectratype drift of T cell receptor alpha and beta chains
摘要
Abstract
Objective To analyze the drift of T cell receptor (TCR) Vα and Vβ gene family CDR3 spectratype in response to ovarian carcinoma cells mediated by an anti-human ovarian carcinoma/CD3 bispecific single-chain antibody (BHL-1), and explore the mechanism of the bispecific single-chain antibody-mediated T cell immune response. Methods Immunoscopic spectratyping technique was used to analyze the TCR repertoire diversity (CDR3 spectratype distribution) of the T cells from 6 healthy donors before and after stimulation of the cells with human ovarian carcinoma in the presence of BHL-1. The predominant usage of TCR a and β chain CDR3 was analyzed after the stimulation, and sequence analysis was performed for the CDR3 region of the monoclonal T cells. Results The spectratypes of Vα and Vβ gene family TCR CDR3 region showed a Gaussian distribution before stimulation of the T cells from the 6 donors. After stimulation of the T cells, CDR3 spectratype drift occurred in the T cells, and some TCR Vα and Vβ families showed an anomalous and oligoclonal expansion. Different CDR3 sequences of the Vα and Vβ gene family TCR were found in the monoclonal T cells stimulated with BHL-1. Conclusion CDR3 spectratype drift occurs in TCR α and β chains of T cells after stimulation with human ovarian carcinoma cells and BHL-1, indicating that the predominant usage of TCR Vα and Vβ families is associated with the specific T cell immune response mediated by BHL-1.关键词
双特异性抗体/抗人卵巢癌/抗人CD3单链双特异性抗体/T细胞受体/互补决定区3/基因扫描Key words
bispecific antibody/ BHL-1/ T cell receptors/ complementarity-determining region 3/ genescan分类
医药卫生引用本文复制引用
罗微,温茜,周明乾,马骊..抗人卵巢癌/抗人CD3单链双特异性抗体介导的αβT细胞CDR3谱系漂移[J].南方医科大学学报,2012,32(7):919-923,5.基金项目
国家自然科学基金(81171539,30972680) （81171539,30972680）
广东省自然科学基金重点项目(S2011020003154) （S2011020003154）
高等学校博士学科点专项科研基金(20114433110002) （20114433110002）
