南方医科大学学报2012，Vol.32Issue(7)：924-927,4.DOI:10.3969/j.issn.1673-4254.2012.07.003
石榴多酚对心肌缺血/再灌注损伤大鼠心功能的保护作用
Protective effects of pomegranate polyphenols on cardiac function in rats with myocardial ischemia/reperfusion injury
摘要
Abstract
Objective To determine the protective effect of pomegranate polyphenols on cardiac function in rats with myocardial ischemia/reperfusion (I/R) injury and explore the possible mechanism. Methods Fifty SD rats were randomized into 5 equal groups, including a sham-operated group, an I/R model group, and 3 pomegranate polyphenol dose groups (150,300, and 600 mg/kg). The rats were subject to a 45-min left main coronary artery occlusion followed by a 180-min reperfusion to induce myocardial I/R injury except for the rats those in the sham-operated group. The cardiac functions were monitored continuously during the experiment. At the end of the reperfusion, arterial blood samples were obtained to measure plasma contents of lactate dehydrogenase (LDH), creatine kinase (CK), malondialdehyde (MDA) and the activities of superoxide dismutase (SOD). Triphenylterrazolium chloride (TTC) staining was used to evaluate the infarct size. Results Compared with the sham-operated group, all the rats with I/R showed significantly decreased left ventricular systolic pressure (LVSP), maximal rates of increase/decrease of the left ventricular pressure (±dp/dtmax) (P<0.01) and significantly increased left ventricular end-diastolic pressure (LVEDP) (P<0.01). Compared with the I/R model group, all the 3 pomegranate polyphenol groups had significantly improved cardiac function (P<0.05), decreased plasma contents of CK, LDH and MDA (P<0.01), increased SOD activities (P<0.01), and obviously reduced infarct size (P<0.01). Conclusion Pomegranate polyphenols can protect the cardiac function of rats with I/R injury probably in association with their actions in enhancing oxygen free radical scavenging activity and decreasing lipid peroxidative damage of the myocardial tissues.关键词
石榴多酚/缺血/再灌注损伤/大鼠/心功能Key words
pomegranate polyphenols/ ischemia/reperfusion injury/ rats/ cardiac function分类
医药卫生引用本文复制引用
董淑英,童旭辉,刘浩,高琴..石榴多酚对心肌缺血/再灌注损伤大鼠心功能的保护作用[J].南方医科大学学报,2012,32(7):924-927,4.基金项目
国家自然科学基金(81000074) （81000074）
安徽省高等学校省级自然科学研究项目(KJ2012B104) （KJ2012B104）
