南方医科大学学报2012，Vol.32Issue(7)：944-947,4.DOI:10.3969/j.issn.1673-4254.2012.07.008
腹腔持续注射血管紧张素-(1-7)对胆管结扎大鼠肝纤维化的抑制作用
Prolonged angiotensin-(1-7) infusion inhibits hepatic fibrosis in rats with bile duct ligation
摘要
Abstract
Objective To observe the inhibitory effect of angiotensin-(1-7) on liver fibrosis induced by bile duct ligation in Tats. Methods Eighteen Wistar rats were randomized into 3 groups and subject to sham operation, bile duct ligation (BDL), or BDL with angiotensin-(1-7) treatment. An osmotic minipump was implanted intraperitoneally for administration of saline in the sham-operated and BDL groups and angiotensin-(1-7) (25 μg·kg-1·h-1) in angiotensin-(1-7) treatment group. After a 4-week treatments, the fibrosis score, Masson staining, and hydroxyproline assay were used to evaluate the level of liver fibrosis in the rats, and irnrnunohistochemistry was used to detect expression of a-smooth muscle actin (a-SMA) in the liver tissue. Results Compared with BDL group, a 4-week treatment with angiotensin-(1-7) following BDL significantly reduced the fibrosis score (2.33 ±0.52 vs 5.17 +0.75), hydroxyproline content (0.36±0.03 vs 0.52±0.04) and a-SMA expression (54.11±17.55 vs 191.84±31.72) in the liver tissue of the rats (P<0.01). Conclusion Prolonged infusion of angiotensin-(1-7) inhibit the formation of hepatic fibrosis in rats following bile duct ligation.关键词
血管紧张素-(1-7)/肝纤维化/α-肌动蛋白/胆管结扎Key words
angiotensin-(1-7)/ hepatic fibrosis/ α-smooth muscle actin/ bile duct ligation分类
医药卫生引用本文复制引用
李旭,宁佐伟,罗薇,张文雍,余常辉,孟莹..腹腔持续注射血管紧张素-(1-7)对胆管结扎大鼠肝纤维化的抑制作用[J].南方医科大学学报,2012,32(7):944-947,4.基金项目
国家自然科学基金(30770974,30871155) （30770974,30871155）
