南方医科大学学报2012，Vol.32Issue(7)：956-959,4.DOI:10.3969/j.issn.1673-4254.2012.07.011
尿路致病性大肠埃希菌外膜蛋白T敲除株的构建及功能评价
Construction and functional studies of uropathogenic E.coli strains with ompT gene knockout
摘要
Abstract
Objective To explore the role of ompT gene in uropathogenic E. coli (UPEC) CFT073 strain in urinary tract infection (UTI). Methods An ompT deletion mutant (COTD) was generated by A Red recombineering in the UPEC CFT073 strain, which was characterized by PCR and sequencing. C57B/L6 mouse models of acute UTI with the mutant and wild-type strains were established to compare the colonization abilities of the two strains in the bladder. The adhesion of CFT073 mutant to human unthelial 5637 cells was also investigated in vitro. Results PCR and DNA sequencing confirmed the loss of ompT gene in the mutant COTD. The in vitro adhesion rate of the mutant strain COTD to 5637 cells was (6.7±2.2)%, significantly lower than that of (8.3 + 1.9)% of the wild-type strain (P<0.05). In the murine models of acute UTI, the mutant strain showed a mean colonization number of about (17±8)×104 cfu, which was significantly lower than that of (7±2)×105 cfu of the wide-type CFT073 strain (P<0.05). Conclusion OmpT gene can be involved in the colonization of UPEC in the bladder tissue and plays an important role in the pathogenesis of UPEC-induced UTI.关键词
尿路致病性大肠埃希菌/外膜蛋白T/尿路感染/定植Key words
uropathogenic E. coli/ outer-membrane protease T/ urinary tract infection/ colonization分类
生物科学引用本文复制引用
赵铁,方幸幸,刘晓露,彭亮,龙敏,张文炳,罗军,曹虹..尿路致病性大肠埃希菌外膜蛋白T敲除株的构建及功能评价[J].南方医科大学学报,2012,32(7):956-959,4.基金项目
国家自然科学基金(30972637) （30972637）
南方医科大学公卫学院院长基金(GW201101) （GW201101）
