南方医科大学学报2012，Vol.32Issue(7)：986-990,994,6.DOI:10.3969/j.issn.1673-4254.2012.07.018
SATB1在不同侵袭潜能肝癌细胞株的表达
Expression of SATB1 in hepatocellular carcinoma cell lines with different invasive capacities
摘要
Abstract
Objective To study the expression of special AT-rich sequence binding protein 1 (SATB1) in hepatocellular carcinoma (HCC) cell lines with different invasive capacities. Methods SATB1 expression was detected using real-time fluorescence quantitative PCR, RT-PCR, Western blotting and immunofluorescence in immortalized liver cell line HL-7702, noninvasive HCC cell lines HepG2 and SMMC-7721, MHCC97L cells with low invasiveness, and highly invasive cell lines MHCC97H and HCCLM3. Results In comparison with HL-7702 cells, all the 5 HCC cell lines showed overexpression of SATB1 mRNA, which was the highest in the highly invasive HCCLM3 and MHCC97H cells, followed by MHCC97L cell line, and then by SMMC-7721 and HepG2 cell lines (P<0.001). The relative expression quantity of SATB1 protein in HepG2, SMMC-7721, MHCC97L, MHCC97H, and HCCLM3 cell lines was 0.271 ±0.002, 0.351 ±0.023, 0.621 ±0.026, 0.878+0.026, and 1.236+0.006, respectively. SATB1 expression level in HCCLM3 cell line was 4.6-fold higher than that in HepG2 cell line (PO.001). SATB1 was found to localize in the cytoplasm and cell nuclei of the 5 HCC cell lines, and the highly invasive HCCLM3 and MHCC97H cell lines showed a strong positive staining for SATB1 in immunofluorescence assay. Conclusion SATB1 expression levels differ distinctly between the HCC cell lines with different invasive capacities and are possibly associated with the metastatic potentials of the cells.关键词
肝细胞肝癌/核基质结合区结合蛋白质1/细胞株Key words
hepatocellular carcinoma/ special AT-rich sequence binding protein 1/ cell lines分类
医药卫生引用本文复制引用
李光辉,杨定华,李湘竑,钟克波,刘萧,毕民平,刘延,廖晓明,林梁..SATB1在不同侵袭潜能肝癌细胞株的表达[J].南方医科大学学报,2012,32(7):986-990,994,6.基金项目
广东省自然科学基金(10151051501000120) （10151051501000120）
