南方医科大学学报2012，Vol.32Issue(8)：1105-1110,6.DOI:10.3969/j.issn.1673-4254.2012.08.07
BRCAl抑制黄体酮刺激乳腺癌细胞增殖和迁移
BRCAl inhibits progesterone-induced proliferation and migration of breast cancer cells
摘要
Abstract
Objective To study the effect of BRCA1 in regulating the proliferation and migration of breast cancer cells stimulated by progesterone. Methods Breast cancer MCF-7 and T-47D cell were transfected with a vector containing the coding sequence of BRCA1 (pFlag-CMV2-BRCAl wt) to induce BRCA1 overexpression or with the empty vector (control). The cells were then stimulated with progesterone, and the cell proliferation and migration were observed using MIT assay and wound healing assay, respectively. The proliferation and migration of MCF-7 cells were also observed following transfection with a small interfering RNA (siRNA) for BRCA1 knockdown or with a scrambled siRNA prior to progesterone stimulation. Results Transfection with the empty vector and with pFlag-CMV2-BRCAl wt prior to progesterone stimulation caused significantly different proliferation rates in MCF-7 cells [(114.4±6.0)% vs (82.1±3.2)%, P<0.05] and in T47D cells [(111.3±4.3)% vs (84.2±3.5)%, P<0.05], resulting also in significantly different cell migration rates (55.9% vs 15.8% in MCF-7 cells and 44.83% ire 10.43% in T-47D cells). Compared to the scrambled siRNA, BRCA1 siRNA transfection prior to progesterone stimulation significantly increased the proliferation rates [(114.4±3.05)% vs (125.3+4.0)%, P<0.05] and migration rate (39.2% vs 69.08%) of MCF-7 cells. The progesterone antagonist RU468 could antagonize the effects of BRCA1 knockdown in enhancing progesterone-stimulated MCF-7 cell proliferation and migration. Conclusions A decreased BRCA1 expression can enhance progesterone-stimulated tumor cell proliferation and migration in sporadic breast cancer.关键词
BRCA1/黄体酮受体/细胞增殖/细胞迁移Key words
BRCA1, progesterone receptor/ cell proliferation/ cell migration分类
医药卫生引用本文复制引用
熊静波,赵嘉佳,彭黎,王宏,梁文颖..BRCAl抑制黄体酮刺激乳腺癌细胞增殖和迁移[J].南方医科大学学报,2012,32(8):1105-1110,6.基金项目
国家自然科学基金(30470673,81071810) （30470673,81071810）
