南方医科大学学报2012，Vol.32Issue(8)：1111-1115,5.DOI:10.3969/j.issn.1673-4254.2012.08.08
支气管肺发育不良新生鼠肺组织甲状腺转录因子及波形蛋白的表达意义
Expression of thyroid transcription factor-1 and vimentin in neonatal mice with bronchopulmonary dysplasis
摘要
Abstract
Objective To investigate the expressions of thyroid transcription factor-1 (TTF-1), the type Ⅱ alveolar epithelial cells (AECII)-specific marker, and vimentin, the fibroblast-specific marker, in the lungs of neonatal mice with bronchopulmonary dysplasia (BPD) and explore the pathogenesis of BFD. Methods Neonatal mice were exposed to hyperoxia to induce BPD, and pathological changes in the lung tissues were examined. At 3, 7,14 and 21 days after the exposure, the protein and mRNA expressions of TTF-1 and vimentin were detected by double-labeled immunofluorescence assay and real-time PCR, respectively. Results Compared with the neonatal mice exposed in normal air, those with hyperoxic exposure showed developmental disorders and collagen deposition in the lung tissues. The protein expression of TTF-1 decreased while vimentin expression increased in the lung tissues, where their co-expression was observed at 14 and 21 days after the exposure. TTF-1 mRNA expression decreased markedly (P<0.05) and vimentin mRNA increased significantly in the lung tissues at 21 days after hyperoxic exposure of the mice (P<0.05). Conclusion Hyperoxia-induced transition of AECH to fibroblasts may play an important role in pulmonary fibrosis in neonatal mice with BPD.关键词
支气管肺发育不良/高氧症/肺泡Ⅱ型上皮细胞/分化Key words
bronchopulmonary dysplasia/ hyperoxia/ typeⅡ alveolar epithelial cells/ differentiation分类
医药卫生引用本文复制引用
陈坤,蒋朴,邓世雄,王念..支气管肺发育不良新生鼠肺组织甲状腺转录因子及波形蛋白的表达意义[J].南方医科大学学报,2012,32(8):1111-1115,5.基金项目
国家自然科学基金(30770950) （30770950）
