南方医科大学学报2012，Vol.32Issue(8)：1127-1130,4.DOI:10.3969/j.issn.1673-4254.2012.08.11
蛋白质组学筛选抑癌基因CCDC19在鼻咽癌中的调控蛋白
Screening of proteins regulated by CCDC19 gene in nasopharyngeal carcinoma using proteomics technology
摘要
Abstract
Objective To screen the proteins under regulation by the candidate tumor suppressor gene CCDC19 using proteomics technology in nasopharyngeal carcinoma (NPC). Method The cellular proteins were extracted from 3D8 NPC cells with CCDC19 overexpression and the control C6 NPC cells. Two-dimensional (2D) gel electrophoresis was employed to compare the protein expression profiles between these two cells, and the differential proteins were identified using peptide mass fingerprinting and database searching. Real-time PCK and Western blotting were used to validate the expression levels of the differential proteins. Results Matrix-assisted laser desorption/time of flight showed that 3 differential proteins, namely FASN, CTSD and PGK1, were down-regulated by -3.28, -1.64, and -6.97 folds, respectively, which were confirmed by real-time PCR and Western blotting. Conclusion FASN, CTSD and PGK1 are probably the target proteins regulated by CCDC19 in NPC.关键词
CCDC19/蛋白质组/鼻咽癌Key words
CCDC19/ proteomics/ nasopharyngeal carcinoma分类
生物科学引用本文复制引用
方唯意,江庆萍,刘真..蛋白质组学筛选抑癌基因CCDC19在鼻咽癌中的调控蛋白[J].南方医科大学学报,2012,32(8):1127-1130,4.基金项目
国家自然科学基金(81071632) （81071632）
广东省育苗工程计划(LYM11102) （LYM11102）
广州市珠江科技新星计划(2011J2200009) （2011J2200009）
