南方医科大学学报2012，Vol.32Issue(8)：1139-1142,4.DOI:10.3969/j.issn.1673-4254.2012.08.14
多柔比星诱导的心衰大鼠心脏胆碱能神经分布及TNF-α表达的变化
Changes of cholinergic nerves and tumor necrosis factor-α in doxorubicion-induced rat failing heart
摘要
Abstract
Objective To investigate the changes of cholinergic nerves in doxorubidn (DOX)-induced rat failing heart and tumor necrosis factor-α (TNF-α) in the heart tissue and serum. Methods Adult Sprague-Dawley rats were randomized into control (n= 10) and DOX-induced chronic heart failure (CHF) groups (n=15), and in the latter group, the rats were given irttraperitoneal injections of 2.5 mg/kg DOX once a week for 6 weeks, with a total cumulative dose of 15 mg/kg. The control rats were injected with normal saline (1 ml/week). Karnovsky-Roots histochemkal staining combined with point counting was used to demonstrate the distribution of cholinergic nerves in the heart. The expression levels of TNF-α in the heart tissue and serum were determined with ELISA. Results Positively stained cholinergic nerves were found in all the rat hearts in the two groups, but in CHF group, the point counts of cholinergic nerves were significantly lower than that of the control group (P<0.01). Compared with the control rats, those with DOX-induced CHF showed elevated levels of TNF-α both in the heart tissue and in the serum (P<0.01). Conclusion In rats with DOX-induced CHF, the parasympathetic nervous system is down-regulated in the failing heart, and the diminished cholinergic anti-inflammatory pathway may play an important role in the progression of CHF.关键词
胆碱能神经/肿瘤坏死因子-α/胆碱能抗炎通路/慢性心力衰竭Key words
cholinergic nerves/ tumor necrosis factor-α/ cholinergic anti-inflammatory pathway/ chronic heart failure分类
医药卫生引用本文复制引用
胥晓丽,曾菊绒,于晓江,弥曼,侯进,孙蕾,李冬玲,臧伟进..多柔比星诱导的心衰大鼠心脏胆碱能神经分布及TNF-α表达的变化[J].南方医科大学学报,2012,32(8):1139-1142,4.基金项目
国家自然科学基金重点项目(30930105) （30930105）
陕西省教育厅科学研究计划项目(112L053) （112L053）
西安医学院青年基金(2007QN01) （2007QN01）
西安医学院扶植项目(10FC14) （10FC14）
