南方医科大学学报2012，Vol.32Issue(8)：1151-1153,3.DOI:10.3969/j.issn.1673-4254.2012.08.17
一种改良的肺微血管内皮细胞培养方法
An improved method for primary culture of mouse pulmonary microvascular endothelial cells
摘要
Abstract
Objective To establish an improved method for culturing primary mouse pulmonary microvascular endothelial cells (PMVECs). Methods An improved tissue block adherent culture method was used to isolate and culture the PMVECs from CS7 mice. The cultured cells were identified by factor Ⅷ-related antigen and CD31 antigen, and the growth of cells cultured using the improved method and the conventional method was compared. Results The cultured primary pulmonary microvascular endothelial cells showed a short fusiform or round morphology, and the cell monolayer displayed a cobble stone-like appearance. The cultured cells were positive for Ⅷ-related antigen and CD31 antigen. The cell growth was accelerated in the cell cultures with the improved method compared with that in conventional cell cultures. Conclusion The improved culture method allows more efficient acquisition of primary mouse PMVECs of a greater purity.关键词
肺微血管内皮细胞/分离培养/鉴定Key words
pulmonary microvascular endhthelial cells/ cell culture/ identification分类
医药卫生引用本文复制引用
胡国栋,陈英华,刘爱华,周国红,康静,蔡绍曦..一种改良的肺微血管内皮细胞培养方法[J].南方医科大学学报,2012,32(8):1151-1153,3.基金项目
国家973计划项目(2012CB518203) （2012CB518203）
广东省科技计划项目(2011B031800245) （2011B031800245）
广东省医学科研基金(B2012218) （B2012218）
南方医院院长基金(2010c002,2011z005) （2010c002,2011z005）
