南方医科大学学报2012，Vol.32Issue(8)：1163-1167,5.DOI:10.3969/j.issn.1673-4254.2012.08.20
Genistein抑制Livin基因对恶性黑色素瘤LiBr细胞凋亡、周期及增殖的影响
Effect of livin gene suppression by genistein on apoptosis, cell cycle and proliferation of malignant melanoma LiBr cells
摘要
Abstract
Objective To observe the effect of livin gene suppression by genistein on apoptosis, cell cycle and proliferation of malignant melanoma LiBr cells. Methods RT-PCR was used to detect the expression of livin mRNA in LiBr cells 48 h after treatment with 40 μmol/L genistein. Flow cytometry with annexin V-FITC/PI double staining was employed to detect cell apoptosis, and the caspase-3 protein expression in the cells following genistein treatment was assayed using Western blotting. The changes in the cell cycle and proliferation of the cells after genistein treatment were examined with flow cytometry with PI staining and MTT colorimetric assay, respectively. Results Genistein can suppress the expression of Livin Gene (87.94% with 40 μmol/L genistein) and induce the apoptosis of LiBr effectively, both in the early and late phases (27.87±5.38% and 11.87± 3.86% respectively). LiBr cells in phase G0/G1 increases notably[G0/G1-(72.11±5.89)% ,S=(14.53±3.47)% ,G2/M=(12.36±2.64%)]. Genistein significantly reduced caspase-3 protein expression and inhibit cell proliferation. Conclusion Genistein can suppress Livin Gene expression, induct LiBr cell apoptosis, hinder cell generation cycle, restrain cell proliferation.关键词
genistein/恶性黑色素瘤/Livin/细胞凋亡/细胞周期/细胞增殖Key words
genistein/ malignant melanoma/ livin/ apoptosis/ cell cycle/ cell proliferation分类
医药卫生引用本文复制引用
杨一飞,王昊,汪京峡,李向花,马梅,杨文斌..Genistein抑制Livin基因对恶性黑色素瘤LiBr细胞凋亡、周期及增殖的影响[J].南方医科大学学报,2012,32(8):1163-1167,5.基金项目
宁夏自然科学基金(NZ08117) （NZ08117）
