发光学报2012，Vol.33Issue(5)：558-561,4.DOI:10.3788/fgxb20123305.0558
荜茇宁与邻硝基苯基荧光酮的荷移反应及其测定
Spectrophotometric Determination of Piperlonguminine Based on The Charge Transfer Reaction Between Piperlonguminine and o-Nitrophenyl Fluorone
摘要
Abstract
A rapid and convenient spectrophotomelric method for the determination of piperlongumi-nine was established in the present work. The method utilized charge transfer reaction between piperlonguminine and o-nitrophenyl fluorone in pH 7. 00 buffer solution. The maximum absorption wavelength of the charge transfer complex was at 515 run and the apparent molar absorptivity was 6.571 ×104 L · mol-1 · cm-1 with a 1 : 1 stoichiometry. Beer's law was obeyed in the range of 0.054 6 ~ 10.92 μg/mL of piperlonguminine with the detection limit of 0.015 3 μg/mL. The reaction mechanism was discussed preliminarily. The proposed method was applied to the biological and urine samples spiked with standard piperlonguminine solution. The recovery of the samples was within the range of 99.8% -100.9% and relative standard deviation (RSD) was 0.90%.关键词
电荷转移反应/邻硝基苯基荧光酮/荜茇苧/分光光度法Key words
charge transfer reaction/ o-nitrophenyl fluorone/ piperlonguminine/ spectrophotometry分类
化学化工引用本文复制引用
敖登高娃,安娜,领小,苗澍..荜茇宁与邻硝基苯基荧光酮的荷移反应及其测定[J].发光学报,2012,33(5):558-561,4.基金项目
国家自然科学基金(20965004) （20965004）
内蒙古自然科学基金(2010BS1203)资助项目 （2010BS1203）
