福建农业学报2012，Vol.27Issue(5)：457-460,4.
鸭副粘病毒弱毒株的选育
Selection of Attenuated Vaccine Strain of Duck Paramyxovirns
摘要
Abstract
An attenuated vaccine strain of Duck Paramyxovirus (DPMVa) was selected and obtained by passage serial within SPF chicken embryo fibroblast (CEF), and some biological property of it were detemined. The DPMVa was non-pathogenic to Muscovy ducks without maternal antibody) and was non-viulence through five serial passages in one-day-old Muscovy ducks; TCID50 to CEF stable between 10-6.0 ~10-6.5 · (0.1 mL)-1. The protection rate of the attenuated strain was above 90% when challenged with a virulent strain of DPMV 7 days after vaccination. The results indicated that attenuated vaccine strain had excellent safety, effective immunogenicity and genetic stability, which could be used as a vaccine against DPMV.关键词
鸭副粘病毒/弱毒株/选育Key words
duck paramyxovirus/attenuated vaccine strain/ selection分类
农业科技引用本文复制引用
程晓霞,陈少莺,朱小丽,林锋强,陈仕龙,王劭,李兆龙..鸭副粘病毒弱毒株的选育[J].福建农业学报,2012,27(5):457-460,4.基金项目
福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y02) （STIF-Y02）
福建省农业科学院科技计划项目(B2010-02) （B2010-02）
