胶体金免疫层析法快速检测水产品中呋喃妥因代谢物残留OA
Gold immunochromatographic assay for rapid determination of nitrofurantoin metabolite residues in aquatic products
采用胶体金免疫层析技术（GICA）测定水产品中的呋喃妥因代谢物残留,并用液相色谱-串联质谱（LC-MS/MS）对实验结果进行确证。结果显示,胶体金免疫层析法的检测限为1.0μg/kg,假阳性率不大于5%,假阴性率为0;对实际样本的检测结果与LC-MS/MS法一致。研究表明,胶体金试纸条法操作简便、快速,成本低,为水产品中呋喃妥因代谢物的残留检测提供了快速筛选方法,而LC-MS/MS法灵敏度高,结果准确,适用于阳性样本的确证和精确定量。
Gold immunochromatographic assay （GICA） was used to determine the nitrofurantoin metabolite （AHD） residues in aquatic products, and also the liquid chromatographytandem mass spectrometry （LC - MS/MS） was used to validate the results. The detection limit of GICA was 1.0 μg/kg. The false positive rate was less 5 % , and false negative rate was 0. The results of the two methods for aquatic products were consistent. The results showed that GICA had the advantage…查看全部>>
吴茂生;何方洋;赵正苗;汪善良;崔廷婷;刘婷婷
福建省海洋环境与渔业资源监测中心,福建福州350003北京勤邦生物技术有限公司,北京102206北京勤邦生物技术有限公司,北京102206北京勤邦生物技术有限公司,北京102206北京勤邦生物技术有限公司,北京102206北京勤邦生物技术有限公司,北京102206
畜牧业
呋喃妥因代谢物水产品胶体金免疫层析法（GICA）液相色谱-串联质谱法（LC-MS/MS）
nitrofurantoin metabolite (AHD)aquatic productsgold immunochromatographic assay ( GICA)liquid chromatographytandem mass spectrometric (LC- MS/MS)
《福建水产》 2012 (4)
290-295,6
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