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胶体金免疫层析法快速检测水产品中呋喃妥因代谢物残留

吴茂生 何方洋 赵正苗 汪善良 崔廷婷 刘婷婷

福建水产2012,Vol.34Issue(4):290-295,6.
福建水产2012,Vol.34Issue(4):290-295,6.

胶体金免疫层析法快速检测水产品中呋喃妥因代谢物残留

Gold immunochromatographic assay for rapid determination of nitrofurantoin metabolite residues in aquatic products

吴茂生 1何方洋 2赵正苗 2汪善良 2崔廷婷 2刘婷婷2

作者信息

  • 1. 福建省海洋环境与渔业资源监测中心,福建福州350003
  • 2. 北京勤邦生物技术有限公司,北京102206
  • 折叠

摘要

Abstract

Gold immunochromatographic assay (GICA) was used to determine the nitrofurantoin metabolite (AHD) residues in aquatic products, and also the liquid chromatographytandem mass spectrometry (LC - MS/MS) was used to validate the results. The detection limit of GICA was 1.0 μg/kg. The false positive rate was less 5 % , and false negative rate was 0. The results of the two methods for aquatic products were consistent. The results showed that GICA had the advantages of convenient operation, provided a rapid method for the screen of AHD in aquatic products, while LC - able for determination of positive samples. rapidity and low cost, which MS/MS was accurate and suit able for determination of positive samples.

关键词

呋喃妥因代谢物/水产品/胶体金免疫层析法(GICA)/液相色谱-串联质谱法(LC-MS/MS)

Key words

nitrofurantoin metabolite (AHD)/aquatic products/gold immunochromatographic assay ( GICA)/liquid chromatographytandem mass spectrometric (LC- MS/MS)

分类

畜牧业

引用本文复制引用

吴茂生,何方洋,赵正苗,汪善良,崔廷婷,刘婷婷..胶体金免疫层析法快速检测水产品中呋喃妥因代谢物残留[J].福建水产,2012,34(4):290-295,6.

福建水产

1006-5601

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