分析测试学报2012，Vol.31Issue(7)：843-847,5.DOI:10.3969/j.issn.1004-4957.2012.07.015
基于银沉积电化学溶出分析的超灵敏甲胎蛋白传感器
Ultrasensitive α-Fetoprotein Immunosensor Based on Electrochemical Stripping Analysis of Silver Deposition
王丽娟 1贾翠娟1
作者信息
- 1. 济宁职业技术学院生物与化学工程系,山东济宁272037
- 折叠
摘要
Abstract
An electrochemical immunosensor was developed for the ultrasensitive measurement of α-fetoprotein based on electrochemical stripping analysis of silver nanoparticles catalytically deposited by nanogold labels. The immunosensor was prepared by covalently immobilizing capture antibodies with glutaraldehyde ( GA) on chitosan-multi wall carbon nanotubes modified glassy carbon electrodes. Through a sandwich-type immunoreaction, antibody-functionalized AuNPs were captured onto the immunosensor surface, and further induced a subsequent silver deposition. The deposited AgNPs were then measured directly by anodic stripping analysis in KC1 solution. Under the optimal conditions, the calibration curve was linear over a-fetoprotein concentration in the range of 0. 02-200 μg/L, the linear regression equation is ΔI = -0. 13 -0. 11c(μg/L) (r =0. 992 8). The detection limit is 8 ng/ L. The electrochemical immunosensor was applied in the determination of a-fetoprotein in three serum samples. The relative standard deviation is no more than 6. 1% . The determination result is identical with the result of electrogenerated chemiluminescence method. The sensor showed acceptable reproducibility, stability and accuracy, indicating a promising potential in clinical diagnose.关键词
α-甲胎蛋白/银沉积/电化学溶出分析/免疫传感器Key words
a-fetoprotein/ silver deposition/ electrochemical stripping analysis/ immunosensor分类
化学化工引用本文复制引用
王丽娟,贾翠娟..基于银沉积电化学溶出分析的超灵敏甲胎蛋白传感器[J].分析测试学报,2012,31(7):843-847,5.
