分析测试学报2012，Vol.31Issue(7)：858-862,5.DOI:10.3969/j.issn.1004-4957.2012.07.018
场放大进样-毛细管区带电泳法高灵敏检测三聚氰胺
Sensitive Determination of Melamine by Field Enhanced Sample Injection -Capillary Zone Electrophoresis Technique
摘要
Abstract
An on-line improved ultraviolet detection method was developed for the determination of melamine in various samples by using water plug coupled with field-enhanced sample injection and capillary zone electrophoresis technique(FESI-CZE). The optimal conditions were as follows; wa-ter plug containing 60% water and 40% acetonitrile, water injection time; 200 s, running buffer; 120 mmol/L NaH2PO4(pH 2.2) - 10% methanol, sample matrix; 0. 10 mmol/L NaH2PO4 (pH 2. 2) - 20% acetonitrile, sample injection voltage: 20 kV, sample injection time: 80 s, separation voltage: 20 kV. Under the optimum conditions, the calibration curve was linear in the range of 10-1 000 μg/L. Compared with that of the traditional CZE method, the sensitivity of FESI - CZE method was improved about 800 times and the LODs( S/N = 3 ) was decreased from 2. 0 mg/L to 2. 5 μg/L. This method was used to detect the melamine in various samples with recoveries of 98% -106% and RSDs(n =4)no more than 5. 1% . This method is sensitive, reliable, economic and pre-treatment-free for complex samples, and it could overcome the shortcoming of low sensibility with ul-traviolet detector.关键词
紫外检测器/毛细管电泳/场放大进样/三聚氰胺Key words
ultraviolet detector/ capillary zone electrophoresis/ field-enhanced sample injection/ melamine分类
化学化工引用本文复制引用
李娟,陈新,刘瑛,商少明,李萌..场放大进样-毛细管区带电泳法高灵敏检测三聚氰胺[J].分析测试学报,2012,31(7):858-862,5.基金项目
国家自然科学基金资助项目(20775028) （20775028）
国家质检总局项目(2010IK183) （2010IK183）
