分析化学2012，Vol.40Issue(8)：1139-1146,8.DOI:10.3724/SP.J.1096.2012.11355
人体血清中的多溴联苯醚、邻苯二甲酸酯和双酚A的连续在线分离及气相色谱-质谱测定
Consecutive On-line Separation and Determination of Polybrominated Diphenyl Ethers, Phthalate Esters and Bisphenol A in Human Serum by Gas Chromatography-Mass Spectrometry
摘要
Abstract
An efficient analytical method was established for the consecutive on-line separation of environmental estrogens in human serum, polybrominated diphenyl ethers (PBDEs), phthalate esters (PAEs) and bisphenol A (BPA) by gas chromatography - mass spectrometry (GC-MS). After denaturation of protein by concentrated hydrochloride acid, extraction by ether, separation by silica flash chromatographic column, several group components including PBDEs, PAEs and BPA in human serum were separated and purified. Then these components were detected by GC-MS in selected ion monitoring mode. The standard calibration curves of PBDEs, PAEs and BPA were linear over the assayed range with correlation coefficients equal or higher than 0. 99. The detected limitations for the targeted compounds ranged from 0. 005 - 0. 05 μg/L for PBDEs, 0. 10 - 0. 83 μg/L for PAEs, and 0. 04 μg/L for BPA. In standard duplicated samples, the relative standard deviations were 2. 8% -10. 9% for PBDEs, 5. 6%-9. 9% for PAEs, and 3.0% for BPA. The'surrogate recoveries in these 7 samples ranged from 74. 8% to 88. 5% for PCB209 (polychloride diphenyl ether 209) in PBDEs, from78. 7% to 97. 0% for DBP-D4 (Dibutyl phthalate-Deutorium 4) in PAEs, and from 76. 3% to 93. 1% for BPA-D16 (Bisphenol A-Deutorium 16) in BPA, respectively. This method for detecting multiple environmental estrogens in human serum is sensitive, high reproducible with satisfied recoveries.关键词
人体血清/多溴联苯醚/邻笨二甲酸酯/双酚A/气相色谱-质谱Key words
Human serum/ Polybrominated diphenyl ethers/ Phthalate esters/ Bisphenol A/ Gas Chromatograph-mass spectrometry引用本文复制引用
邵敏,陈永亨,李晓宇..人体血清中的多溴联苯醚、邻苯二甲酸酯和双酚A的连续在线分离及气相色谱-质谱测定[J].分析化学,2012,40(8):1139-1146,8.基金项目
本文系国家环保部公益性行业科研专项(No.201109001-08)资助 （No.201109001-08）
