分析化学2012，Vol.40Issue(8)：1236-1240,5.DOI:10.3724/SP.J.1096.2012.20343
基于核酸切割酶与脱氧核酶的荧光循环放大系统检测铅(Ⅱ)
Amplified Fluorescence Detection of Pb2+ Using pb2+-dependent Combined with Nicking Enzyme-Mediated Enzymatic Recycling Amplification
摘要
Abstract
A fluorescence sensing system was developed for the detection of Pb2+ with excellent sensitivity and selectivity based on 8-17E DNAzyme and nicking endonuclease mediated fluorescence cyclic amplification strategy. In the presence of Pb2+ , the 8-17E DNAzyme catalyzed the cleavage of RNA substrate. Subsequently, the partial substrate strand would dissociated from DNAzyme and hybridized with molecuar beacon (MB) , resulting in the restoration of the fluorescence signal and the formation of the double-stranded recognition site for nicking endonuclease (Nt. BbvCD. After the Nt. BbvCI mediated cleavage of MB probes, the released partial substrate strand could then hybridize with another MB probe and be re-used for the second cycle of cleavage. Eventually, each target-induced partial substrate strand can trigger many cycles of cleavage to achieve the cyclic amplified fluorescence detection of Pb2+. This new design both avoids the modification on 8-17E DNAzyme and substrate, and significantly improves the sensitivity with a detection limit down to 1. 0× 10-10 mol/L. Moreover, it also exhibited satisfactory selectivity for Pb2+ detection, even in the presence of 2 times of Zn2+ and 5 times of other interferential metal ions. Furthermore, the applicability of this proposed method for Pb2+ detection in environmental samples was demonstrated with a Pb2+ recovery from 96. 1 % to 108%. These advantages endow the sensing strategy with a great potential for the facile on-site and real-time Pb2+ sensing from a wide range of real samples.关键词
铅(Ⅱ)/脱氧核酶/核酸切割酶/荧光循环放大检测Key words
Lead(Ⅱ)/ DNAzyme/ Nicking endonuclease/ Cyclic amplified fluorescence detection引用本文复制引用
赵永席,齐林,杨卫军,魏帅,王亚玲..基于核酸切割酶与脱氧核酶的荧光循环放大系统检测铅(Ⅱ)[J].分析化学,2012,40(8):1236-1240,5.基金项目
本文系中国烟草总公司陕西省公司研究基金项目资助 （）
