高等学校化学学报2012，Vol.33Issue(7)：1456-1461,6.DOI:10.3969/j.issn.0251-0790.2012.07.016
新型聚乙二醇-聚L-赖氨酸负载四(对磺酸基偶氮苯基-4-氨基磺酰基)铝(Ⅲ)氯酞菁的纳米光敏剂的合成及离体光动力活性
Synthesis of Poly( ethylene glycol)-poly(L-lysine) Diblock Copolymer Incorporating Tetra-(p-sulfoazophenyl-4-aminosulfonyl) phthalocyanine Chloride Aluminum ( Ⅲ ) Polyion Nanoparticles and Its in vitro Photodynamic Therapy Efficacy
摘要
Abstract
A novel tetra-(p-sulfoazophenyl-4-aminosulfonyl) phthalocyanine aluminum( Ⅲ) chloride (S-AlPc) was synthesized and characterized by elemental analysis, IR, 1H NMR and ESI-MS. Polyion nanoparticles (S-AlPc/PbP) loaded with S-AlPc were formed through electrostatic self-assemble by negatively charged sulfonate groups with positively charged PLL of the poly(ethylene glycol)-poly(L-lysine) amphiphilic diblock copolymer (PEG-b-PLL). The diameter of S-AlPc/PbP was about 10-20 nm. The photophysical properties of 5-AIPc/PbP and S-AlPc were compared by UV-Vis, steady-state and time-resolved fluorescence spectroscopy. The time-depended uptake amounts of S-AlPc and 5-AIPc/PbP in the human umbilical cord blood endothelial cells(HUVEC) were studied. The uptake amounts of S-AlPc/PbP by HUVEC reached maximum about one hour earlier than that of the 5-AlPc. The phototoxicity of S-AlPc and S-AlPc/PbP was evaluated by MTT method. The inhibition rate of S-AlPc/PbP was also significantly increased. These results obviously indicated that S-AlPc/PbP is a potential 3rd generation photosensitizer for photodynamic therapy.关键词
酞菁铝/聚合物纳米粒子/光动力活性/聚乙二醇-聚L-赖氨酸/光敏剂Key words
Phthalocyanine aluminium/ Polyion nanoparticles/ Photodynamic therapy efficacy/ Poly(ethylene glycol)-poly(L-lysine) diblock copolymer/ Photosensitizer分类
化学化工引用本文复制引用
马冬冬,林萍萍,陈莉莉,王瑜华,贺丹丹,陈婉玲,张甜甜,陈奎治,彭亦如..新型聚乙二醇-聚L-赖氨酸负载四(对磺酸基偶氮苯基-4-氨基磺酰基)铝(Ⅲ)氯酞菁的纳米光敏剂的合成及离体光动力活性[J].高等学校化学学报,2012,33(7):1456-1461,6.基金项目
国家自然科学基金(批准号:60978071,20604007)、福建省自然科学基金(批准号:2011J01029,2010J01322)和卫生部科学研究基金(批准号:WKJ2008-2-61)资助. （批准号:60978071,20604007）
