广东医学2012，Vol.33Issue(18)：2746-2749,4.
单酰甘油脂肪酶在原发性肝细胞肝癌中的表达及意义
The expression and clinical significance of MAGL in human primary hepatocellular Carcinoma
摘要
Abstract
Objective To evaluate the expression and clinical significance of monoacylglycerol lipase ( MAGL ) in primary hepatocellular carcinoma ( HCC ). Methods The MAGL expression of cancer tissues and the adjacent non -tumor tissues from 50 HCC cases, and of hepatic tissues from 16 normal livers, were assessed with Real - Time PCR, im-munohistochemistry and Western Blot. The correlation between the MAGL expression and pathological characteristics were also analyzed. Results ( 1 ) According to immunohistochemetry, MAGL was mainly expressed in nucleus in cancer tissues , while cytoplasmic expression was dominated in the adjacent non - tumor tissues and normal hepatic tissues. In addition, there were significant differences in MAGL expression among cancer tissues, adjacent non - tumor tissues and normal tissues ( P < 0. 05 ), furthermore, the MAGL expression was significantly higher in poorly differentiated liver cancer tissue than that in well differentiated liver tissues ( P < 0. 05 ). (2) According to real - time PCR, the MAGL mRNA was significantly evaluated in either poor differentiated or high differentiated cancer tissues, when comparing with that in normal hepatic tissues ( P <0. 05 ). ( 3 ) According to Western Blot, strong positive MAGL expression was observed in cancer tissues, while weak positive MAGL expression was revealed in adjacent non - tumor tissues and normal tissues. Furthermore, the MAGL expression was significantly up - regulated in either poor or high differentiated hepatic cancer tissues when comparing with that in both adjacent non - tumor and normal hepatic tissues ( P < 0. 01 ). Conclusion The up - regulation of MAGL is positively correlated with the malignancy of primary hepatocellular carcinoma, which probably plays an important role in invasions and metastasis of cancer cells.关键词
单酰甘油脂肪酶/肝细胞肝癌/脂代谢Key words
monoacylglycerol lipase/ hepatocellular carcinoma/ lipid metabolism引用本文复制引用
秦易,连峥嵘,覃飞,王晓波,龚建平..单酰甘油脂肪酶在原发性肝细胞肝癌中的表达及意义[J].广东医学,2012,33(18):2746-2749,4.基金项目
重庆市医学科研计划项目(重点项目)(编号:2011-1-123) （重点项目）
