国际医药卫生导报2012，Vol.18Issue(13)：1844-1848,5.DOI:10.3760/cma.j.issn.1007-1245.2012.13.002
黄芩苷对D半乳糖诱导衰老大鼠拮抗脑老化糖基化氧化应激及胰岛素抵抗作用
The effects of baicalin on inhibiting brain aging, glycation-oxidative stress and insulin resistance in D-galactose-induced aging rats
摘要
Abstract
Objective To observe the effects ofbaicalin ( Bai ) on non-enzymatic glycation ( NEG ) -oxidative stress,activity of aldose reductase,insulin resistance ( IR )and spatial seeking ability in D-galactose ( D-gal ) -induced senile rats.Methods Except the blank control,a rat model induced by D-gal [ 150 mg.kg-1,intraperitoneal injection ( ip ),qd×70 d] was established.28 days after treatment with D-gal,the rat models were randomly divide into 5groups ( 15 rats for each goup ):model control,metformin ( Met,150 mg·kg-1 ),and pimagedine ( PG,150 mg·kg-1 ),Bai high dose group( 300 mg·kg-1 )and low dose group ( 150 mg·kg-1 ); then ip D-gal and different drugs or distilled water ip-treated qd for 42 days.In the end of the experiment,the contents of fasting blood glucose ( FBG ),fruetosamine ( FRA ),glycosylated hemoglobin ( HbAIc ),advanced glycation end-products ( AGEs ),insulin,and malondialdehyde ( MDA ) were analyzed,the activity of aldose reductase ( AR ) and superoxide dismutase ( SOD ) were determined,insulin sensitivity index ( ISI ) was counted and spatial seeking ability by Morris water maze test was selected.Results As compared with blank control,the concentration of FBG,FRA,HbA lc,AGEs,insulin and MDA increased ( P＜ 0.01 ),SOD activity declined ( P＜ 0.01 ),AR activity enhanced ( P＜ 0.01 ),ISI reduced ( P＜ 0.01 ),and spatial seeking ability significantly declined in the D-gat-induced rats; as compared with the D-gal-induced aging rats,the contents of FBG,FRA,HbAIc,AGEs and insulin were reduced,AR activity was inhibited,SOD activity was elevated,and spatial seeking ability was significantly increased by using Met,PG,and Bai at high or low dose ( P ＜ 0.05 or P ＜ 0.01 ).Conclusions Baicalin can antagonize insulin resistance,improve insulin sensitivity,inhibit formation of glycation products-oxidative stress and activity of rate-limiting enzyme AR in polyoi pathway,suppress inflamnatory reaction,and improve spatial seeking ability in D-gal-induced aging rats.The effects of baicalin reveals a dose-effect relationship.关键词
黄芩苷/D-半乳糖/衰老/糖基化-氧化应激反应/胰岛索抵抗/醛糖还原酶Key words
Baicalin/D-galactose/Aging/Glycation-oxidative stress/Insulin resistance/Aldosereductase引用本文复制引用
钟成,潘竞锵,吕俊华,郑琳颖,李博萍..黄芩苷对D半乳糖诱导衰老大鼠拮抗脑老化糖基化氧化应激及胰岛素抵抗作用[J].国际医药卫生导报,2012,18(13):1844-1848,5.基金项目
广东省中医药管理局滚动资助项目(301005.302003) （301005.302003）
广州市中医药、中西医结合课题(2009A012) （2009A012）
