国际医药卫生导报2012，Vol.18Issue(13)：1947-1948,2.DOI:10.3760/cma.j.issn.1007-1245.2012.13.046
高效液相色谱法测定根痛平滴丸中芍药苷和葛根素的含量
High performance liquid chromatographic method for determinntion of contents of paeoniflorin and puerarin in root pain ping pills
摘要
Abstract
Objective To detect the contents of paeoniflorin and pueratin in root pain ping pills by using high performance liquid chromatography.Methods Agela TMC 18 column was used and water methanol acetonitrile was used as mobile phase,with a flow rate of 0.96 ml / min,column temperature of 30°C,and detection wavelength of 250 nm,to determine the contents of paeoniflorin and puerarin in root pain ping pills; and the related analysis was conducted.Results Paeoniflorin was not less than 0.80 mg and puerarin was not less than 0.28 mg in each pill.Paeoniflorin in 59～ 1150 mg / L and puerarin in 9～180 mg/ L had a good linear relation.Conclusions High performance liquid chromatography for detecting drug content is simple to operate and accurate and has good reproducibility and a satisfied recovery rate.It has a good effect for quality control of root pain ping pillat.关键词
根痛平滴丸/芍药苷/葛根素/高效液相色谱法/含量Key words
Root pain ping pills/Paeoniflorin/Puerarin/High performance liquid chromatography/Content引用本文复制引用
庞惠民,郁相云,钟建华..高效液相色谱法测定根痛平滴丸中芍药苷和葛根素的含量[J].国际医药卫生导报,2012,18(13):1947-1948,2.
