果树学报2012，Vol.29Issue(4)：544-549,6.
根癌农杆菌介导的CG1-400-RNase基因转化创制锦橙转基因再生植株
Creation of transgenic plants in Jincheng orange （Citrus sinensis） with CG1-4OO-RNase gene by Agrobacterium-mediated transformation
摘要
Abstract
[Objective] The objective of the study was to quickly and effectively obtain the citrus cuhivars with seedless trait by using genetic engineering. Chimeric ribonuclease gene （Barnase） driven by seed- specific promoter （CG1-400） for reducing seed number, was introduced into ‘Jineheng＇ orange, an important commercial and seeded citrus variety in China, [Method] by Agrobacterium-mediated transformation and using epicotyl segments as explants. [Result] To screen the tansformants effectively, different concentration of kanamycin was used at different developmental stages since the sensitivities of different tissues and organs to kanamycin were different. The results showed that 362 resistant shoots were obtained when 50 rag·L^（-1） Kan was added into the shoot-induction medium, 28 of which were PCR-positive buds after those were cultivated on the shoot-elongation medium with 100 mg·L^（-1） Kan. Later twenty two plantlets were obtained by inducing to root or grafting in vitro. [Conclusion] PCR and Southern blot analysis showed that single copy of CG1-400-RNase gene was integrated into two transgenic plants. The study will facilitate the breeding of transgenic citrus lines with seedless trait.关键词
柑橘/根癌农杆菌/CGl-400-RNase基因Key words
Citrus/Agrobacterium/CG1-400-RNase gene分类
农业科技引用本文复制引用
谭彬,李鼎立,徐世晓,郭文武..根癌农杆菌介导的CG1-400-RNase基因转化创制锦橙转基因再生植株[J].果树学报,2012,29(4):544-549,6.基金项目
国家863计划（2011AA100205）,国家自然科学基金项目 （）
