果树学报2012，Vol.29Issue(4)：569-576,8.
南疆杏品种自交不亲和S—RNase基因型的鉴定
Identification of self-incompatibility S-RNase genotypes for apricot cultivars in South of Xinjiang area
摘要
Abstract
[Objective] In order to identify S-RNase genotypes associated with self-incompatibility of apricot cultivated in Xinjiang, [Method] three pairs of consensus primer EM-PC2consFD＋EM-PC3con- sRD, PaCons 11 -F＋PaCons 11 -R and PruC2＋PruC5 were used to amplify genome DNAs of the leaves from 27 main apricot cultivars, which had been published for S-RNase in Prunus. The specific fragments were cloned and sequenced, and then the nucleotide acid sequences were blasted on GenBank. [Result] The results showed： 54 gene fragments were amplified from 27 apricot cultivars successfully. Searches for ho- mology with our sequences publicly available DNA databases revealed 4 S-RNase gene fragments had been deposited in the GenBank and designated as S24 （588 bp）, S14 （708 bp）, S23 （972 bp）, Szl （971 bp） ,respectively, and 15 S-RNase gene fragments were novel S-RNase genes, temporarily named as S40 （572 bp） （ accession number： HQ342870）, S41 （401 bp） （accession number： HQ342871）, S42 （357 bp） （accession number： HQ342872）, S43 （493 bp） （accession number： HQ342873）, S44 （657 bp） （ accession number： HQ342874）, S45 （1 294 bp） （ accession number： HQ3428570）, S46 （830 bp） （accession number： HQ342876）, S47 （575 bp） （accession number： HQ342877）, S48 （594 bp） （accession number： HQ342878）, S49 （653 bp） （accession number： HQ342879）, S50 （659 bp） （accession number： HQ342880）, S51 （768 bp） （accession number： HQ342881）, S66（1 520 bp） （aceession number： HQ342882）, S65 （594 bp） （accession number： JQ327151）, S66 （709 bp） （accession number： JQ327152）. S-genotypes of the 27 apricot cultivars of Xinjiang were identified including Sz3S14 for Wan- shuhuanna, Hexieke, Beixinnaer, S52S14 for Qiaoerpang, Mantouyulvke, S14S47 for Keziaqia, Yuandanx- ing, Lajiaoxing, S23S48 for Shaeheheiyexing, Kuehetuoyong, Anjianghuanna, S5zS23 for Suogejianali, S52Ssl for Dongxing, S5zS5o for Suluke, S52S4o for Kabakehuanna, S43S41 for Jagedamayishang, Hongdauke, Cui- jianali, Kalahuanna, Kezijianali, S45S42 for Maolaxiao, S45S46 for Tuohuti, S45S44 for Shailaikeyulvke, S49S24 for Luerletuoyong, Huangrouyouxing, S21S65 for Saimaiti, S52S66 for Xiaobaixing. [Conclusion] S-genotypes of the 27 aprieot eultivars in Xinjiang were identified, which provided academic basis for choosing suitable pollination cultivars for eommercial apricot cultivation.关键词
杏/自交不亲和/S-RNase基因/S-基因型Key words
Apricot/Self-incompatibility/S-RNase gene/S-genotypes分类
农业科技引用本文复制引用
姜新,曹晓艳,王大江,冯建荣,刘月霞,樊新民..南疆杏品种自交不亲和S—RNase基因型的鉴定[J].果树学报,2012,29(4):569-576,8.基金项目
国家自然科学基金资助项目 （）
科技部“863”计划 （）
