果树学报2012，Vol.29Issue(4)：589-592,I0001,5.
欧李离体叶片再生体系的建立
Adventitious shoot regeneration from leaf in vitro of Prunus humilis
摘要
Abstract
[Objective] In order to lay the foundation for cultivar improvement of stone fruits, genetic transformation and functional verification of bioteehnology breeding, we studied adventitious shoot regen- eration system of Prunus humilis Bge. using leaf explants. [Method] Different basic media and growth reg- ulator combinations were tested for the adventitious shoot regeneration system. [Result] The results showed that shoot proliferation was sensitive to basic media, and medium MS （modified） was better than MS, F14 and WPM. For multiplication culture, the multiplication coefficient was 5.6 in MS （modified）＋ NAA0.1 mg＇L^（-1）＋BA 0.2 mg·L^（-1）.The optimum plant growth regulators for adventitious shoot regeneration were MS （modified） ＋ TDZ 4.0 mg·L^（-1） IBA 0.2 mg·L^（-1） with regeneration rate of 63.10%.The average amount of regenerated buds for each explant was 4.9. TDZ was more effective than BA and ZT in inducing adventitious shoot regeneration. Dark treatment for 10 days could increase the rate of shoot regeneration. The best medium for rooting was 1/2MS （modified） ＋ IBA3.0 mg·L^（-1） with the rooting rate of 93.3%. [Conclusion] The best regeneration efficiency was obtained with TDZ plus IBA in MS（modified）medium.关键词
欧李/不定芽/再生Key words
Prunus humilis Bge./Adventitious buds/Regeneration分类
农业科技引用本文复制引用
姚婷,张开春,闫国华,王晶,张晓明..欧李离体叶片再生体系的建立[J].果树学报,2012,29(4):589-592,I0001,5.基金项目
北京市科技新星计划 （）
北京市科技新星计划 （）
公益性行业（农业）科研专项经费 （）
北京市农林科学院科技创新能力建设专项 （）
北京市农林科学院一般项目 （）
