果树学报2012，Vol.29Issue(4)：598-604,F0004,8.
石榴2种外植体再生方法在遗传转化研究中的优势比较
Comparison of advantages of two kinds of explant regeneration methods for studies on genetic transformation in pomegranate
摘要
Abstract
[Objective] The leaf and stem segments of ＇Tunisia soft-seed＇ pomegranate were used as the explants for genetic transformation research. [Method] The effects of different sterilization time and different growth regulators on the in vitro leaf and stem segment regeneration were studied, and the advantages of two regeneration systems were compared. [Results] The results showed that it was easier for stem segments to be sterilized than for the leaf, but the green and compact callus from leaves were more likely to be obtained and then more adventitious buds differentiated. The callus from stem segments were crisp, and the adventitious buds were less. The adventitious buds from the stem segment callus grew more quickly, but the time consuming of leaf regeneration system was shorter. The adventitious buds could be induced from a few emerald green leaves, which could be used for transformation, but the adventitious buds could not be induced directly from stem segments.There was not any significant difference between stem and leaf explants in callus sensitivity to antibiotics. [Conclusions] As a result, the leaf callus regeneration system might be more appropriate for the genetic transformation research of pomegranate.关键词
‘突尼斯软籽’石榴/再生体系/遗传转化Key words
‘Tunisia soft-seed' pomegranate/Callus regeneration system/Genetic transformation分类
农业科技引用本文复制引用
陈延惠,谭彬,李洪涛,胡青霞,叶霞,孟海军,冯建灿..石榴2种外植体再生方法在遗传转化研究中的优势比较[J].果树学报,2012,29(4):598-604,F0004,8.基金项目
：河南省科技攻关项目 （）
郑州市科技攻关项目 （）
