果树学报2012，Vol.29Issue(4)：644-649,I0002,7.
拮抗放线菌MY一4的分离、鉴定及其对果蔬病原菌的抑制作用
Isolation and identification of actinomyce strain MY-4 and its antagonistic activity against pathogenic fungi of fruits and vegetables
摘要
Abstract
[Objective] The purpose of the study is to identify the antagonistic actinomyce strain MY-4 isolated from the surface of Shatang mandarin, and to evaluate the application potential of the strain for the biocontrol of fruit and vegetable diseases. [Method] The taxonomic identification of the strain was carried out based on the morphology, physio-biochemical characteristics and the 16S rDNA sequence analysis, and the antagonistic activity of strain MY-4 was determined by confronting incubation on the PDA plates and the mycelium growth rate method. [Result] Strain MY-4 was identified as Streptomyces albtdus. The results showed that strain MY-4 and its cell-free fermentation filtrate exhibited extensive antagonistic ef- fect on 16 fruit and vegetable pathogenic fungi. The strain exhibited the strongest antibiosis capacity with an inhibitory zone up to 17.08 mm against mycelia growth of Colletotrichum musae. Meanwhile, its culture filtrate showed the strongest antibiosis capacity against Penicillium digitatum and reduced mycelium growth by up to 98.82%. [Conclusion] These findings show that Streptomyces albulus strain MY-4 has high activity, broad antifungal spectrum, and is expected to develop into a new biocontrol agent.关键词
小白链霉菌/拮抗作用/病原菌/生物防治Key words
Streptomyces albulus/Antagonistic activity/Pathogenic fungi/Biological control分类
农业科技引用本文复制引用
罗建军,李辉,耿鹏,胡美英..拮抗放线菌MY一4的分离、鉴定及其对果蔬病原菌的抑制作用[J].果树学报,2012,29(4):644-649,I0002,7.基金项目
国家自然科学基金项目 （）
广东省科技计划项目 （）
