广东药学院学报2012，Vol.28Issue(4)：396-399,4.DOI:10.3969/j.issn.1006-8783.2012.04.010
利用ISSR分子法标记鉴别新会茶枝柑
Identification of Citrus Reticulata cv.Chachiensis by ISSR molecular markers
摘要
Abstract
Objective To identify Citrus Reticulata cv. Chachiensis from Xinhui by ISSR molecular markers from different species of Citrus Reticulata. Methods 28 ISSR primers were analyzed by PCR with total DNA extracted from fresh leaves of 16 samples of Citrus Citrus Reticulata cv. Chachiensis , reticulata ' Shiyueju' , reticulata ' Yangshan' ,reticulata' Gong' and reticulata' Lu'. Results Two ISSR primers amplified from Citrus Reticulata cv. Chachiensis products showed different electrophoresis bands with other species of Citrus Reticulata. Conclusion ISSR method provides a quick, reliable molacular marker technique for identification of Citrus Reticulata cv. Chachiensis.关键词
茶枝柑/ISSR/鉴别Key words
Citrus Reticulata cv. Chachiensis/ ISSR/ identification分类
医药卫生引用本文复制引用
邓锋,林向华,梁蔚阳..利用ISSR分子法标记鉴别新会茶枝柑[J].广东药学院学报,2012,28(4):396-399,4.基金项目
广东省中医药管理局资助项目(2010252) （2010252）
