广东药学院学报2012，Vol.28Issue(4)：410-414,5.DOI:10.3969/j.issn.1006-8783.2012.04.014
不同来源的穿心莲药材HPLC指纹图谱研究
Study on HPLC fingerprint of Andrographis paniculata ( Burm.f.) Nees of different sources
摘要
Abstract
Objective To establish an HPLC fingerprint for quality control of Andrographis paniculata ( Burm. F. ) Nees. by cluster analysis. Methods The chromatographic fingerprints were obtained by HPLC with Welch Ultimate XB-C18 (250 mm × 4. 6 mm,5 μm) . The mobile phase was acetonitrile-water solution with gradiem elution. The detection wavelength was set at 226 nm. The flow rate was 1. 0 mL · min-1 and the column temperature was maintained at 25 ℃. The similarity assay of A. paniculata of different sources was carried out to evaluate their quality by Traditional Chinese Medicine fingerprint similarity evaluation system (2004A Edition) ,and hierarchical cluster analysis was performed by SPSS software. Results Ten main characteristic peaks were selected in the standard fingerprint, and the similarity of samples from different GAP bases was over 0. 91 ,and the similarity of 70% samples from different pharmacies was over 0. 86. About 12 batches of A. paniculata from different sources were classified into two groups through the results of hierarchical cluster analysis. Conclusion The method with good reproducibility is simple and accurate, which can be used as a quality control method for A. paniculata.关键词
穿心莲/HPLC/指纹图谱Key words
Andrographis paniculata (Burm. F. )Nees. / HPLC/ chromatographic fingerprint分类
药学引用本文复制引用
谢郁峰,李斌,陈卓瀚,陈荣海,郑海英,郭丽冰..不同来源的穿心莲药材HPLC指纹图谱研究[J].广东药学院学报,2012,28(4):410-414,5.基金项目
国家自然科学基金(81073045) （81073045）
广东药学院2010年学生创新实验项目 （）
