广东药学院学报2012，Vol.28Issue(4)：430-434,5.DOI:10.3969/j.issn.1006-8783.2012.04.019
白芍总苷对NAFLD模型大鼠肝脏保护作用的抗氧化机制
The antioxidative mechanism of total glucosides of paeony on protection against the fatty liver in NAFLD rats
赵汝霞 1郑琳颖 2潘竞锵 2陈依雨 1吕俊华 1周永标 3潘卫松 3肖瑛3
作者信息
- 1. 暨南大学药学院,广东广州510632
- 2. 广州市中医中药研究所,广东广州510130
- 3. 广州市药品检验所,广东广州510160
- 折叠
摘要
Abstract
Objective To investigate the antioxidative mechanism of total glucosides of paeony (TGP) on protection against nonalcoholic fatty liver disease ( NAFLD) rats. Methods fifty-four SD rats were randomly divided into normal group ( n = 12) and model group (n =42) . The normal rats were raised by standard animal diet,while the model rats were fed with high-fat diet and 10% fructose for 6 weeks. Two rats then were taken out from each group and extracted liver for HE staining to observe hepatocyte pathological changes. Model rats were randomly divided into 4 groups, including nonalcoholic fatty liver group, metformin group (200 mg · kg-1 ) , high-dose TGP group ( 200 mg · kg-1 ) and low-dose TGP group (100 mg · kg-1 ). All rats were treated for 4 weeks. The activities of serum alanine aminotransferase (ALT) ,aspartate aminotransferase (AST) ,glutathione S-transferase (GST) and liver index were detected. The activities of superoxide dismutase (SOD) ,nitric oxide synthase (NOS) ,glutathione peroxidase (GSH-PX) ,and the contents of malondialdehyde ( MDA ) , nitric oxide (NO) in serum and liver tissue were measured. The contents of serum fructose amine (FMN) and advanced glycation endoproducts (AGEs) were also measured. Results Compared with normal rats, the activities of serum ALT, AST, GST and liver index were increased in model rats ( P < 0. 01 ). Meantime, the activities of SOD and GSH-PX were decreased (P <0. 01 ) ,but the activities of NOS and contents of MDA,NO,FMN and AGEs were increased (P<0.01) in model rats. After treatment with TGP for 4 weeks,the activities of serum ALT,AST,GST and liver index were significantly decreased (P < 0. 01 ; P < 0. 05). The activities of SOD and GSH-PX were increased ( P < 0. 01 ; P < 0. 05 ) , but the activities of NOS and the contents of MDA, NO, FMN and AGEs were decreased ( P < 0. 01 ; P < 0. 05 ) . Conclusion TGP possess protective effect from the fatty liver in NAFLD rats,and the possible mechanism may be related to increasing antioxidative ability.关键词
白芍总苷/非酒精性脂肪肝/抗氧化/氧化应激Key words
total glucosides of paeony/ nonalcoholic fatty liver disease/ antioxidation/ oxidative stress分类
医药卫生引用本文复制引用
赵汝霞,郑琳颖,潘竞锵,陈依雨,吕俊华,周永标,潘卫松,肖瑛..白芍总苷对NAFLD模型大鼠肝脏保护作用的抗氧化机制[J].广东药学院学报,2012,28(4):430-434,5.
