神经损伤与功能重建2012，Vol.7Issue(4)：241-243,3.DOI:10.3870/sjsscj.2012.04.003
嗅鞘细胞体外CFSE染色的标记方法研究
CFSE Fluorescence Staining of Olfactory Ensheathing Cells in vitro
摘要
Abstract
Objective: To establish a method of CFSE labeling olfactory ensheathing cells (OECs) at appropriate final concentration in vitro. Methods: OECs were labeled by 2, 5, or 10 μM CFSE in vitro. The positive labeling rate, cell viability, growth and morphological charac teristics and proliferation of OECs were detected by flow cytometry, trypan blue staining and fluorescence microscopy respectively. Results: More than 99% of the OECs were CFSE-labeled at the three concentrations (2, 5, 10 μM) investigated. The OECs labeled with 2 μM CFSE had the highest viability and the maximum proliferation rate. However, the fluorescence intensity was significantly decreased with time. Conclusion: CFSE can be used to label OECs with optimized concentration of 2 μM.关键词
CFSE/嗅鞘细胞/荧光/标记/体外Key words
CFSE/ olfactory ensheathing cells/ fluorescence/ label/ in vitro分类
医药卫生引用本文复制引用
刘娜,唐颖馨,倪厚杰,张誉,刘永明,唐洲平..嗅鞘细胞体外CFSE染色的标记方法研究[J].神经损伤与功能重建,2012,7(4):241-243,3.基金项目
国家自然科学基金(No.30570628,30770751,81171089) （No.30570628,30770751,81171089）
