神经损伤与功能重建2012，Vol.7Issue(4)：244-248,5.DOI:10.3870/sjsscj.2012.04.004
细胞周期抑制剂对糖氧剥夺诱导神经元凋亡的保护机制研究
Protective Effects of Cell Cycle Inhibitor on Neuronal Apoptosis Induced by Oxygen-glucose Deprivation in vitro
摘要
Abstract
Objective: To explore the protective effect and potential mechanism of roscovi-tine on rat cortical neuronal apoptosis induced by oxygen-glucose deprivation (OGD) in vitro. Methods.- Primary rat cortical neuronal cultures were randomly divided into 6 groups; control group, the groups of 3 h, 6 h, 12 h, 24 h reoxygenation after 1 h of OGD and roscovitine (100 μM) treated group. The expressions of phospho-Rb (p-Rb) and E2F1 were detected by western blot. Immunofluorescence staining was used to detect percentage of neurons with p-Rb- positive and TUNEL-positive, and the correlation between them was analyzed. Results: When compared with the control group,the expressions of p-Rb and E2F1 in each group after OGD were increased (P<0. 05) and peaked at 12 h after OGD, which were decreased in the roscovitine treated group (P<0. 05). Roscovitine significantly reduced the expression of p-Rb and decreased neuronal apoptosis at 12 h after OGD (P<0. 01). A majority of apoptotic neurons of TUNEL staining had a co-localization with p-Rb staining. Conclusion; Roscovitine could reduce neuronal apoptosis, perhaps by way of inhibiting phosphorylation of Rb and transcription factor E2F1 after an ischemia/hypoxia injury in vitro.关键词
神经元/糖氧剥夺/凋亡/细胞周期Key words
neuron/ oxygen-glucose deprivation/ apoptosis/ cell cycle分类
医药卫生引用本文复制引用
余樱,喻志源,王伟,张兆辉..细胞周期抑制剂对糖氧剥夺诱导神经元凋亡的保护机制研究[J].神经损伤与功能重建,2012,7(4):244-248,5.基金项目
国家自然科学基金重点项目(No.81030021) （No.81030021）
武汉大学青年教师自主创新项目(No.111089) （No.111089）
