南方农业学报2012，Vol.43Issue(6)：727-732,6.DOI:10.3969/j:issn.2095-1191.2012.06.727
甘蔗MAP激酶基因SoMAPK4克隆与生物信息学分析
Cloning and bioinformatic analysis of Mitogen-activated protein kinase gene SoMAPK4 in sugarcane
摘要
Abstract
[Objective]The present experiment was conducted to clone the full-length sequence of mitogen-activated protein kinase (MAPK) in sugarcane to explore mechanism of stress resistance in sugarcane. [ Method ]The total RNA was extracted from leaves of sugarcane variety ROC22 and the cDNA was synthesized by gene racer kit. Based on the cDNA sequences of the conserved regions of plant MAPK genes, a pair of primers was designed and synthesized for cloning the full-length sequence of a novel member of MAPK gene family by RACE reaction. The bioinformatics of the cloned MAPK gene was also analyzed. [Result]The cloned mitogen-activated protein kinase gene from sugarcane showed 92.6% homolo-gy with ZmMAPK4, and it was named as SoMAPK4 (accession No. JQ062930), which cDNA full-length was 1499 bp with a polyA tail of 23 bp, containing a 5'-untranslated region (UTR) of 218 bp and a 3'-untranslated region (UTR) of 213 bp. The cDNA contained an ORF of 1128 bp encoding a protein of 376 amino acids with a calculated molecular weight of about 43.5 kDa and a pl of 5.51. The SoMAPK4 protein contained 11 sub-domains of protein kinase with serin/ threonine specificity and phosphorylation TEY motif of MAP kinase. [ Conclusion ] A novel MAPK gene SoMAPK4 was cloned. The SoMAPK4 may be involved in many signal transduction activities for various stress reactions, and is the key factor for studying signal transduction during sugarcane biotic and abiotic stresses.关键词
甘蔗/MAP/基因克隆/SoMAPK4/生物信息学Key words
sugarcane/ MAP/ gene cloning/ SoMAPK4/ bioinformatics分类
农业科技引用本文复制引用
李粲,刘芳,邱永福,李鸣,李容柏,滕峥,刘开雨,桑洪玉,卢双楠,方位宽,梁朝旭,刘晓静,何姗珊..甘蔗MAP激酶基因SoMAPK4克隆与生物信息学分析[J].南方农业学报,2012,43(6):727-732,6.基金项目
广西科学基金项目(桂科自0990182,桂科基0778006-4,桂科青0832059,2011GXNSFF018002,2011GXNSFD018021) （桂科自0990182,桂科基0778006-4,桂科青0832059,2011GXNSFF018002,2011GXNSFD018021）
广西农业科学院博士后基金项目(桂农科博2009013) （桂农科博2009013）
广西农业科学院基本业务专项项目(201107Z基,G2010003) （201107Z基,G2010003）
广西农业科学院公益性维持项目(桂农科2012GW13) （桂农科2012GW13）
南宁市科学研究与技术开发计划项目(201102026B) （201102026B）
