南方农业学报2012，Vol.43Issue(6)：738-743,6.DOI:10.3969/j:issn.2095-1191.2012.06.738
Ac/Ds系统及其在油菜突变体库构建中的应用策略
Ac/Ds transposon system and its application strategy on construction of rape mutants pool
摘要
Abstract
With the completion of the rape (Brassica campestris) genome sequence and its subsequent improvement, the study on functional genomics in rape has come into focus, therefore, how to construct high throughput mutants pool is the key affecting its advances. Ac/Ds (Activator/Dissociation) system is a promising method to construct insertional mutants pool of heterologous plant such as Oryza sativa and Arabidopsis thaliana, however it rarely has information related to rape. In this paper, difficulties and advantages of using Ac/Ds system, characteristics and mechanism of Ac/Ds system were reviewed. Combined with the current achievements on rice and Arahidopsis thaliana, the improvement strategies for construction of rape mutants pool by Ac/Ds system were analyzed further, and the application prospects of Ac/Ds system used in rape functional genomics were summarized.关键词
油菜/Ac/Ds系统/突变体库/应用策略Key words
rape/ Ac/Ds system/ mutants pool/ application strategy分类
农业科技引用本文复制引用
洪林,闫晓红,刘正富,魏文辉..Ac/Ds系统及其在油菜突变体库构建中的应用策略[J].南方农业学报,2012,43(6):738-743,6.基金项目
国家自然科学基金项目(30671312) （30671312）
国家科技部科技支撑计划项目(2010GB2F100391) （2010GB2F100391）
