南方农业学报2012，Vol.43Issue(7)：895-900,6.DOI:10.3969/j:issn.2095-1191.2012.07.895
24个荸荠品种遗传多样性RAPD分析
Genetic diversity analysis of 24 water chestnut (Eleocharis tuberosa Schulut) cultivars using RAPD markers
摘要
Abstract
[Objective]The present experiment was conducted to study the genetic diversity and relationship amongst different water chestnut (Eleocharis tuberosa Schulul) cultivars to provide reference for breeding water chestnut. [Method] Random amplified polymorphism DNA (RAPD) molecular markers were used to assess the genetic diversity amongst 24 water chestnut (Eleocharis tuberosa Schulut) cultivars using RAPD primers and cluster analysis was performed. [Result] Out of 100 primers, 15 primers with clear bands and polymorphism were selected and 83 reproducible DNA bands were amplified, of which 61 bands (73.5%) were polymorphic. The coefficient of genetic similarity in different cultivars ranged from 0.0976 to 0.6757 with an average of 0.3048. UPGMA cluster analysis results showed that all the cultivars were clustered into five groups, and the fourth group could be divided into two subgroups. The wild species belonged to the fifth group alone. [Conclusion ]Narrow genetic diversity and close genetic relationship presented amongst 24 water chestnut (Elmcharis tuherosa Schulut) cultivars, certain genetic differences still existed among those cultivars come from different.关键词
荸荠/RAPD/遗传多样性/聚类分析Key words
water chestnut (Ehocharis tuberosa Schulut)/RAFD/genetic diversity/clustering analysis分类
农业科技引用本文复制引用
江文,蔡炳华,陈丽娟,欧昆鹏,郭畅,杨丽涛,李杨瑞..24个荸荠品种遗传多样性RAPD分析[J].南方农业学报,2012,43(7):895-900,6.基金项目
国家科技支撑计划项目(2012BAD27B01) （2012BAD27B01）
广西科学研究与技术开发计划项目(桂科攻10100008-5) （桂科攻10100008-5）
广西农业科学院科技发展基金项目(2009003,桂农科2012JZ04) （2009003,桂农科2012JZ04）
