南方农业学报2012，Vol.43Issue(7)：901-906,6.DOI:10.3969/j:issn.2095-1191.2012.07.901
海岛棉部分引进和自选品种遗传多样性的SRAP分析
Genetic diversity analysis of introduced and self-bred sea-island cotton (Gossypium barbadense L.) varieties based on SRAP markers
摘要
Abstract
[Objective]The present experiment was conducted to investigate the genetic diversity of sea-island cotlon germplasm accessions and provide references for application of sea-island cotton germplasm accessions and breeding elite varieties. [Method]Sequence related amplified polymorphism (SRAP) marker technology was used to analyze genetic diversity of 168 sea-island cotton cultivars. Sixteen pairs of primer combinations with clear band and high polymorphism were selected from 224 pairs of primer combinations. [Result]In total, 129 polymorphic loci were detected by sixteen pairs of SRAP primers, and each primer combination amplified 3 to 12 loci with an average of 8.06 loci. UPGMA cluster analysis results showed that the genetic similarity coefficient of sea-island cotton accessions ranged from 0.27 to 0.89, and the 168 sea-island cotton germplasm accessions were divided into four groups at coefficient threshold of 0.49: the 1st group mainly involved introduced foreign varieties at early stage, which contained Egyptian and Central Asian varieties, the 2nd group mainly included a series of Xinku varieties, part of Central Asian varieties and American Pima varieties, the 3nd group mainly contained a series of Xinhai varieties, part of Central Asian varieties and Soviet Union varieties, the 4th group just only has Giza 85 in it. [Conclusion]The genetic similarity coefficient of sea-island cotton cultivars ranged widely, which showed great difference and rich generic diversity among the 168 accessions.关键词
海岛棉(Gossypium barbadense L.)/SRAP/遗传多样性/聚类分析Key words
sea-island cotton/SRAP/genetic diversity/cluster analysis分类
农业科技引用本文复制引用
武路云,刘方,靳明凯,孔祥军,王坤波,周瑞阳..海岛棉部分引进和自选品种遗传多样性的SRAP分析[J].南方农业学报,2012,43(7):901-906,6.基金项目
农业部资源保护项目(NB2012-2130135-29B) （NB2012-2130135-29B）
中央级公益性科研院所基本科研业务费专项项目(SJA1201) （SJA1201）
