南方农业学报2012，Vol.43Issue(7)：955-960,6.DOI:10.3969/j:issn.2095-1191.2012.07.955
广西南方水稻黑条矮缩病毒及水稻齿叶矮缩病毒的分子检测
Molecular detection of southern rice black-streaked dwarf virus and rice ragged stunt virus in Guangxi
摘要
Abstract
[Objective] The current research was conducted to explore molecular biological methods for detecting southern rice black-streaked dwarf virus (SRBSDV) and rice ragged stunt vims (RRSV), understand the distribution of two viruses in Guangxi, and lay the foundation for controlling the two viruses in production. [Method]RT-PCR technique was used to detect the two viruses from rice, weeds and vector insects samples gathered from different regions of Guangxi. Fibrous cellulose powder (CF-11) was used to extract dsRNA from rice leaves and stems. dsRNA electrophoregram was observed on 1.5% agarose gel under ultraviolet light. [Result]A 850 bp sequence of SRBSDV was obtained from rice, Echinochloa cwsgalli, Miscanthus fioridulus, Leersia hexandra and Sogatella furciferu by RT-PCR, and a 700 bp sequence of RRSV from rice and Nihparvata higens. SRBSDV was detected from Miscanthus Qoriduius and Leersia hexandra for the first time. Eight bands of dsRNA were delected from rice infected by SRBSDV alone and by both SRBSDV and RRSV, but the sizes of dsRNA hands were different. Five bands of dsRNA were detected from rice infected by RRSV. A-mong the detected 181 rice samples from different regions of Guangxi, excepl rice samples from Beihai, Guigang and Wuzhou, SRBSDV were detected and reached 44.8 percent of the total samples. Except rice samples from Hezhou, Guigang, Yulin, Laibin and Wuzhou, RRSV were detected and reached 21.5 percent of the total samples, in which 11.0% of rice samples were infected by both SRBSDV and RRSV. [Conclusion]The special primer designed could be used in RT-PCR to detect SRBSDV and RRSV. dsRNA extracted from rice samples could rapidly and visually identify rice samples infected by SRBSDV, RRSV or both. Thanks to the designed special primer, two new hosts to SRBSDV. Mis-canfhus floridulu (Labnll.) Warb and Leersia hexandra, were discovered for the very first time.关键词
南方水稻黑条矮缩病毒/水稻齿叶矮缩病毒/RT-PCR/dsRNA/广西Key words
SRBSDV/RRSV/RT-PCR/dsRNA/Guangxi分类
农业科技引用本文复制引用
刘红艳,章友爱,廖咏梅,李子玲,王凯学,陈保善..广西南方水稻黑条矮缩病毒及水稻齿叶矮缩病毒的分子检测[J].南方农业学报,2012,43(7):955-960,6.基金项目
广西自然科学基金项目(2010GXNSFA013066) （2010GXNSFA013066）
