南方农业学报2012，Vol.43Issue(7)：1049-1053,5.DOI:10.3969/j:issn.2095-1191.2012.07.1049
一株从家蚕体内分离获得的微孢子虫GXM2的生物学特性
Biological characteristics of a strain of microsporidia GXM2 isolated from silkworm
摘要
Abstract
[Objective]The present study was conducted to investigate the pathogenicily and classification features of a strain of microspofidia (GXM2) isolated from silkworm in order to provide basis for comprehensively controlling silkworm pebrine. lMethod]GXM; infected symptom of silkworm was observed, the ingeslive infectivity of GXM, to silkworm was detemwncd by use of 50% infectious concenlration (IC50) and the semen infection raie of CXM2 was investigated under microscope, spore morphology, serological reaction, life cycle and internal structure were observed under optical microscope and transmission electron microscope. [ Result ] GXM2 infected symptom of silkworm was in acute morbidity, short course of disease(6-9 days), and no ecdysis or half ecdysis, which was similar to physiological diseases. IC50 of GXM2 was 2.76× 105 spores/mL, 4.8 times of that of N.b. The semen infection rate of GXM2 was 1.19%. GXM2 spore was oval in shape and (2.49±010) cm×(4.19±0.]5) μm in size, and did not condense in scrum of N.b immune antibody- During the life cycle of GXM2, uninucleat and binuclear coexisted, propagated in binary fission, then formed lwo kinds (uninuclear and binuclear) of mature spores. Innerstructure of GXM2 was quite, different from N.h, with 9 to 11 coils of polar filament in spores. [Conclusion]GXM1 was strongly infective and harmful to Bombyx mori. GXM2 and Nsrma bnmbycis (N.b) were different species and different genus. Classified inspection and processing technologies should be adopted to further improve the technology system of silkworm pebrine control.关键词
微孢子虫/GXM2/家蚕/致病性/生物学特征Key words
microspofidia/GXM2/Bombyx mori/pathogenieity/biological characteristics分类
农业科技引用本文复制引用
黄旭华,祁广军,汤庆坤,潘志新,罗梅兰,蒋满贵,朱方容,黄深惠..一株从家蚕体内分离获得的微孢子虫GXM2的生物学特性[J].南方农业学报,2012,43(7):1049-1053,5.基金项目
广西科学研究与技术开发项目(桂科攻0718004-2A) （桂科攻0718004-2A）
广西自然科学基金项目(桂科自1013072,桂科自0832179) （桂科自1013072,桂科自0832179）
