广西医科大学学报2012，Vol.29Issue(1)：19-23,5.
血管内皮细胞对卵巢癌淋巴结定向转移细胞侵袭能力的影响
EFFECT OF VASCULAR ENDOTHELIAL CELL ON INVASIVENESS CAPABILITY OF HUMAN OVARIAN CARCINOMA CELL WITH DIRECTIONAL HIGHLY LYMPHATIC METASTASIS
摘要
Abstract
Objective: To establish the condition medium of culture cell system with SKOV3-PM4 and HU-VEC and to study their invasiveness and movement capability. Methods: To collect the culture medium of SKOV3PM4 cells as SKOV3-PM4 condition medium and the culture medium of HUEVC as HUEVC condition medium. The growth curve of the cells was determined by MTT assay. The ultrastructure and morphological changes of cells in the condition medium cultivation were detected by Hematoxylin and eosin staining. The change of each cells invasiveness and migration capability were detected by healing assay, tr-answell chamber model and gelatin zymography. Results; As compared to the single culture SKOV3-PM4, the cells which cultured in HUVEC condition medium showed the increase of pseudopodia and grows more quickly, the healing assay and transwell chamber model showed that invasiveness and migration capability enhancement. Compared to the single culture HUVEC, the cells which cultured in SKOV3-PM4 condition medium showed the morphological significantly changed and vacuolization in the cytoplasm and grew more slowly, the healing assay and transwell chamber model showed that invasiveness and migration capability enhancement. Gelatin zymography assay showed that MMP-2 low-expressed in SKOV3-PM4 and didn't expressed in HUVEC in RPMI1640 medium and high-expressed in SKOV3-PM4-HUVEC in the co-culture system. Conclusion: The biological characteristics and invasiveness capability of SKOV3-PM4 show significantly changes in HUVEC condition medium of culture, it may related to vascular endothelial secretion of VEGF, MMP-2 and other factors.关键词
卵巢癌/人脐血管内皮细胞/条件培养基交互培养/侵袭转移Key words
ovarian carcinoma/ human umbilcical venous endothelial cells/ the condition medium of culture/ invasiveness and migration分类
医药卫生引用本文复制引用
高婷,黎丹戎,李力,张玮,阮和云,冠潇..血管内皮细胞对卵巢癌淋巴结定向转移细胞侵袭能力的影响[J].广西医科大学学报,2012,29(1):19-23,5.基金项目
国家自然科学基金资助项目(No.81060218),教育部高等学校博士学科点专项科研基金资助项目(No.20050598002),广西自然科学基金资助项目(No.桂科自0728193) （No.81060218）
