广西医科大学学报2012,Vol.29Issue(1):50-52,3.
自制混合血浆建立血凝仪室内质控的可行性探讨
FEASIBILITY DISCUSSION ON ESTABLISHING OF INTERNAL CONTROL OF BLOOD COAGULATION ANALYZER BY USING SELF-MADE MIXED BLOOD PLASMA
摘要
Abstract
Objective: To explore the establishment of internal control of blood coagulation analyzer by using self-made mixed plasma. Methods: The fresh mixed blood plasma was collected from the patients with normal coagulation, divided into EP centrifuge tubes and then restored in low temperature refrigerator ( — 20 'O for conservation. Two tubes were removed from the refrigerator and placed at room temperature per day. After thawing unaffectedly, one was used as normal blood plasma, another was diluted with normal saline by three folds as abnormal blood plasma. PT, APTT, Fib were assayed simultaneously in the normal and abnormal self-made blood plasma, matching quality control substance and clinical specimens. Results: In three months, there was no statistically significance difference between the cryopreserved self-made mixed blood plasma and the raw self-made mixed blood plasma on the results of PT, APTT and Fib ( P >0. 05). In the third month, PT, APTT and Fib analysis showed the results of the cryopreserved self-made mixed plasma were consistent with those of the matching quality control substance in L-J quality control graph, they had good correlation ( r>0. 95). Conclusion: The cryopreserved self-made mixed plasma can be used as internal control on blood coagulation analyzer. Two tubes were removed per day, one can be used as normal control and the other diluted with normal saline by three folds can be used as abnormal control.关键词
混合血浆/质控品/血凝仪/室内质控Key words
the mixed blood plasma/ quality control substance/ blood coagulation analyzer/ internal control分类
医药卫生引用本文复制引用
唐劲光,林发全,郭谊,李世生..自制混合血浆建立血凝仪室内质控的可行性探讨[J].广西医科大学学报,2012,29(1):50-52,3.基金项目
广西卫生厅科研课题(桂卫科研Z2009110) (桂卫科研Z2009110)