广西医科大学学报2012，Vol.29Issue(4)：499-501,3.
ZHX2-GST融合蛋白原核表达载体的构建和鉴定
CONSTRUCTION AND IDENDIFICATION OF EUKARYOTIC PROKARYOTIC EXPRESSION VECTOR ZHX2-GST FUSION PROTEIN
摘要
Abstract
Objective: To construct the prokaryotic expression vector of GST-ZHX2 fusion gene in Esche-richia coli, and assay the GST-ZHX2 fusion protein. Methods:ZHX2 gene RNA was extracted from normal human liver tissue, reverse transcriptased and amplified into full length cDNA by RT-PCR, cloned into the GST tagged prokaryotic expression vector PGEX-4-1. The fusion gene GST-ZHX2 -was expressed in E-coli. BL 2KDE3) induced by IPTG and the GST-ZHX fusion protein was assayed by SDS-PAGE. Results: The sequence of the cloned ZHX2cDNA was corrected and the molecular weight of expression production assayed by SDS-PAGE -was 118 ku, coincided -with GST-ZHX2. Conclusion: The prokaryotic expression vector of GST-ZHX2 fusion gene is constructed successfully, and the fusion protein can be used in clinical and experiment research.关键词
ZHX2/谷胱甘肽转移酶(GST)/融合蛋白/原核表达Key words
ZHX2/ GST/ fusion protein/ prokaryotic expression分类
医药卫生引用本文复制引用
杜杨君,文剑明,吕自力..ZHX2-GST融合蛋白原核表达载体的构建和鉴定[J].广西医科大学学报,2012,29(4):499-501,3.基金项目
广西青年科学基金资助项目(No.0728057) （No.0728057）
