工业微生物2012，Vol.42Issue(4)：13-20,8.DOI:10.3969/j.issn.1001-6678.2012.04.003
基于蛋白表达分析的干酪乳杆菌ATCC393交互胁迫保护机制
Protective mechanism of Lactobacillus casei ATCC393 against multiple stresses by proteomics analysis
摘要
Abstract
To investigate the physiological response of lactobacillus casei ATCC 393 under cross stresses, the two-dimensional gel electrophoresis and the iTRAQ technology were used to analyze the proteome changes before and after the cross-stress treatment. It is found that the main proteins of Lactobacillus casei ATCC393 distribute in the range of PI 4-7, and great changes take place after the acid pre-adaption. Based on the iTRAQ analysis before and after acid pre-adaption as well as the related lethal treatments, the up-regulation of acid-induced heat stress shock proteins (dan K, dna J) , oxidative stress shock proteins ( mutS, rec O) and some metabolic enzymes were confirmed to be the key factors to improve the multi-stress resistance of cells. Moreover, the high expression of GTP cyclohydrolase I and GMP synthase after acid pre-adaptation may be related to the induction of this process. Results in this study will help to improve the stress resistance of industrial strains against serious environments during the fermentation and processing. Furthermore, it also provides a novel way to enhance the performance of industrial strains by regulating certain functional components with renewed biology information.关键词
干酪乳杆菌/交互胁迫/蛋白质组学/二维电泳/iTRAQKey words
Lactobacillus casei/ cross-stress/ proteomics/ two-dimensional gel electrophoresis/ iTRAQ引用本文复制引用
张娟,薛峰,吴重德,堵国成,陈坚..基于蛋白表达分析的干酪乳杆菌ATCC393交互胁迫保护机制[J].工业微生物,2012,42(4):13-20,8.基金项目
国家高技术研究发展计划(863项目,2011AA100901) （863项目,2011AA100901）
国家自然科学基金重点项目(20836003) （20836003）
国家自然科学基金(30900013). （30900013）
