工业微生物2012，Vol.42Issue(4)：38-42,5.DOI:10.3969/j.issn.1001-6678.2012.04.007
大孔树脂分离纯化蛹虫草深层发酵液中虫草素的工艺研究
Purification of cordycepin from fermentation broth of Cordyceps militaris with macroporous resin
摘要
Abstract
Ten kinds of macroporous resins were compared for separating cordycepin from Cordyceps militaris in order to select the suitable resin, and the resin of H103 was chosen as the best one. Then the adsorption conditions and elution conditions of cordycepin by using H103 were investegated. The results showed that when pH value was 9.0, concentration of sample was 0.2 mg · mL-1 and the flow rat was 2 BV · h-1, the H103 resin had good adsorption solution capacity. As a better result, the cordycepin could be eluted from H103 resin with 40% ethanol.关键词
蛹虫草/深层发酵/虫草素/大孔树脂Key words
Cordyceps militaris/ fermentation broth/ cordycepin/ macroporous resin引用本文复制引用
陈涛,沈健增,蔡宇杰,廖祥儒,岳翠翠,张大兵..大孔树脂分离纯化蛹虫草深层发酵液中虫草素的工艺研究[J].工业微生物,2012,42(4):38-42,5.基金项目
国家自然科学基金(21045007) （21045007）
江苏省科技创新与成果转化(重大科技支撑与自主创新)专项引导资金(BY2010117) （重大科技支撑与自主创新）
中央高校基本科研业务费专项资金资助(JUSRP21120). （JUSRP21120）
