工业微生物2012，Vol.42Issue(4)：54-59,6.DOI:10.3969/j.issn.1001-6678.2012.04.010
种间双亲灭活原生质体融合选育高产Monacolin K红曲菌
Breeding of Monascus spp.with high monacolin K production by interspecific protoplast fusion of inactivated parental strains
摘要
Abstract
In order to obtain high monacolin K producing strains, Monascus albidus H2 and Monascus Jumeus 9908 were used as parents. M. albidus H2 with hygromycin resistance gene was acquired by Agrobacterium-medisAed transformation and had high monacolin K production by liquid state fermentation using glycerol as main carbon source, and M. Jumeus 9908 could convert rice into monacolin K in solid state fermentation. The protoplasts of parents were inactivated by heat and ultraviolet respectively, and fused with PEG as fusion agent. Fusants with hygromycin resistance gene were selected for further fermentation and compared with parental strains. Two fusants, F12-11 and F13-2 were obtained. It was revealed the fusant F12-11 produced more monacolin K by solid state fermentation with rice, the monacolin K yield was 8.73 mg/g, increased by 100.23% and 48.98% respectively compared with M. albidus H2 and M. Jumeus 9908; the monacolin K yield of the fusant F13-2 was 1752.46 mg/L in liquid state fermentation with glycerol, increased by 32.98% and 1979.33% respectively compared with M. albidus H2 and M. Jumeus 9908.关键词
红曲菌/原生质体融合/Monacolin KKey words
Monascus/ protoplast fusion/ monacolin K引用本文复制引用
刘月,王璐,许赣荣..种间双亲灭活原生质体融合选育高产Monacolin K红曲菌[J].工业微生物,2012,42(4):54-59,6.
