工业微生物2012，Vol.42Issue(4)：60-64,5.DOI:10.3969/j.issn.1001-6678.2012.04.011
纺锤芽孢杆菌CGMCC1347发酵生产异丁香酚单加氧酶的条件优化
Optimization of fermentation conditions for isoeugenol monooxygenase from Bacillus fusiformis CGMCC1347
摘要
Abstract
Fementaition conditions for Bacillus fusiformis CGMCC1347 producing isoeugenol monooxygenase were optimized through single and orthogonal experiments. The suitable fermentation conditions were determined as follows: 2.49 g/L vanillin was obtained from 20 g/L isoeugenol with the cells cultured at initial pH 7.5, 37 ℃, 180 r/min for 16 h in the fermentation culture containing urea 1 g/L, K2HP04 2 g/L, com steep liquid 55 g/L, and MgS04 · 7H2O 1 g/ L. Under the optimized conditions, the isoeugnol monooxygenase activity reached 3.79 U/L.关键词
异丁香酚单加氧酶/香草醛/纺锤芽孢杆菌/发酵Key words
isoeugenol monooxygenase/ vanillin/ Bacillus fusiformis/ fermentation引用本文复制引用
赵丽青,肖向东,孙志浩,方佳茂,陈伟滨..纺锤芽孢杆菌CGMCC1347发酵生产异丁香酚单加氧酶的条件优化[J].工业微生物,2012,42(4):60-64,5.基金项目
深圳大学基础研究科研项目(No.00035226). （No.00035226）
