华北农学报2012，Vol.27Issue(3)：50-54,5.
黄瓜黄色子叶突变体遗传及相关AFLP标记
Genetic Analysis and AFLP Markers Associated to the Yellow Cotyledon Trait in Cucumber
摘要
Abstract
With a F2and BC1 population derived from F1 of B180H( yellow cotyledon female) and Q12( green cotyledon male) was used as materials to study the inheritance of yellow cotyledon color and molecular marker associated to the cotyledon yellow trait. The results indicated that the yellow cotyledon trait was controlled by a pair of recessive gene,and the green cotyledon trait was in the mode of incomplete dominant inheritance. The polymorphism between yellow and green cotyledon parents of cucumber were studied using BSA method and AFLP technology. 1 024 AFLP markers were screened, and a co-dominant marker E-AG/M-AAG was obtained. Sequencing of the polymorphic fragment indicated that their lengths were 258 bp and 257 bp, respectively, and they were orthologous sequences with an insertion /deletion or mutant of only one nucleotide. The yellow cotyledon F2 plant possessed the 258 bp fragments, and the green cotyledon F2 plants possessed the 257 bp fragments or possessed both two fragments. The marker was closely linked to the cucumber cotyledon yellow color trait-related gene,and the marker was confirmed to be 96. 74% accurate by F2 individuals. The trait was a useful marker for hybridization and purity identification of F, hybrids.关键词
黄瓜/子叶/遗传规律/分子标记/AFLPKey words
Cucumber/Cotyledon color/Inheritance/Molecular marker/AFLP分类
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王惠哲,李淑菊,杨瑞环,管炜..黄瓜黄色子叶突变体遗传及相关AFLP标记[J].华北农学报,2012,27(3):50-54,5.
