华北农学报2012，Vol.27Issue(3)：55-61,7.
火把梨14-3-3基因的克隆与序列分析
Isolation and Expression Analysis of a 14-3-3 Gene from Pyrus pyrifolia Nakai cv.Huobali
摘要
Abstract
Based on the EST sequence encoded the 14-3-3, gene-specific primer was designed and used to ?obtain the full-length cDNA of a novel 14-3-3 gene from Pyrus pyrifolia Nakai cv. Huobali in Yunnan province with the method of rapid amplification of cDNA ends (RACE). This novel gene was named as Ppl4-3-3. Ppl4-3-3 is 1 107 bp in length with an ORF of 786 bp,a 5'-untranslated region (UTR) of 84 bp,and a 3'-UTR of 237 bp,and the ORF encodes a predicted polypeptide of 261 amino acids. The Ppl4-3-3 shares higher homology with the known 14-3-3 proteins,and possesses the basic stucture of 14-3-3 proteins. A phylogenetic analysis of the relationship of the newly identified Ppl4-3-3 with some known 14-3-3s from other species grouped the Ppl4-3-3 into the class of non-e 14-3-3s. Ppl4-3-3 is abundantly expressed in pericarps of Huobali regardless received sunlight or not,and also expression in the young leaves. Isolation and expression analysis of Ppl4-3-3 in this study laid the groundwork for further studying on function of Ppl4-3-3 .关键词
火把梨/RACE/RT-PCR/14-3-3Key words
Pyrus pyrifolia Nakai cv. Huobali/ Rapid amplification of cDNA ends /RT-PCR/ 14-3-3分类
生物科学引用本文复制引用
王光勇,刘迪秋,李旻,饶健,孙兵召,丁元明..火把梨14-3-3基因的克隆与序列分析[J].华北农学报,2012,27(3):55-61,7.基金项目
云南省应用基础研究面上项目(2008ZC036M) （2008ZC036M）
