华北农学报2012，Vol.27Issue(3)：72-74,3.
MC4R与Leptin、MC3R和CCKAR基因的互作研究
The Interaction of MC4R with Leptin ,MC3R and CCKAR Genes
摘要
Abstract
To study the potential relationships among the four genes associated with intake, recombinant method was used to build a Melanocortin receptor-4 (MC4R) gene eukaryotic expression vector. The expression vector was transfected into fibroblasts by liposome method. Transient transfection after 24 h, Real-time PCR method was used to detect and analyze Leptin,MC3R and CCKAR mRNA expression changes. The results showed that, in fibroblasts, overexpression of MC4R gene, Leptin, MC3R and CCKAR mRNA levels were not significantly changed. Presumed that although these four genes are all involved in the regulation of animal feed intake, but they are likely to be regulated by a different pathway.关键词
猪/成纤维细胞/MC4R/Real-time PCR/过表达Key words
Pig/Fibroblasts/MC4R/Real-time PCR/Overexpression分类
生物科学引用本文复制引用
张冬杰,汪亮,刘娣..MC4R与Leptin、MC3R和CCKAR基因的互作研究[J].华北农学报,2012,27(3):72-74,3.基金项目
转基因生物新品种培育重大专项(2008ZX08006-003) （2008ZX08006-003）
现代农业产业技术体系(CARS-36) （CARS-36）
